Presence of catecholamines and serotonin in the rat vestibule

The concentrations of norepinephrine (NE), dopamine (DA) and its metabolites DOPAC and HVA, and serotonin (5-HT) and its metabolite 5-HIAA, were quantified in the rat vestibule. For this purpose, homogenates of vestibules, of albino and pigmented rats, were analyzed using HPLC with electrochemical detection. Vestibules of pigmented rats showed higher DOPAC and HVA concentrations than those of albino rats, and male pigmented rats also showed significantly more DA than male albino rats. These results could indicate that the rate of DA metabolism in vestibules was higher in pigmented than in albino rats. The vestibular concentrations of NE and 5-HT did not differ significantly between the two strains. In contrast, 5-HIAA concentration was higher in vestibules of pigmented rats than in those of albino rats, suggesting an increased 5-HT metabolism for the former strain. Differences in monoamine concentrations between the two sexes of the same strain were scarce. Only, a higher HVA concentration in vestibules of females could indicate a higher DA metabolism.