Glutathione protects a human insulinoma cell line from tumor necrosis factor-alpha-mediated cytotoxicity

被引：7

作者：

Cavallo, MG

Monetini, L

Valente, L

Barone, F

Beales, P

Russo, M

Pozzilli, P

机构：

[1] UNIV LONDON ST BARTHOLOMEWS HOSP MED COLL, DEPT DIABET & METAB, LONDON EC1A 7BE, ENGLAND

[2] UNIV ROMA LA SAPIENZA, IST CLIN MED 2, ROME, ITALY

[3] UNIV ROMA LA SAPIENZA, DIPARTIMENTO MED SPERIMENTALE, I-00185 ROME, ITALY

来源：

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH | 1997年 / 27卷 / 01期

关键词：

glutathione; human insulinoma; tumor necrosis factor-alpha; diabetes immunotherapy;

D O I：

10.1007/BF02827241

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

It is postulated that glutathione acting as a free oxygen radical scavenger may protect beta-cells from cytokine-mediated cytotoxicity in insulin-dependent diabetes. In this study the effect of glutathione in preventing the cytotoxic damage mediated by tumor necrosis factor-a in vitro towards a human beta-cell line (CM insulinoma) was investigated. CM cells were exposed in vitro to tumor necrosis factor-alpha tumor necrosis factor-alpha plus glutathione or glutathione alone at different concentrations. The resulting cytotoxicity was measured using a colorimetric assay. Glutathione significantly reduced the cytotoxicity mediated by tumor necrosis factor-alpha in a dose-dependent fashion (P<0.001). These results suggest a protective effect of glutathione on beta-cell cytotoxicity induced by tumor necrosis factor-alpha and encourage the use of glutathione in trials aimed at reducing the beta-cell damage occurring in insulin-dependent diabetes.

引用

页码：44 / 47

页数：4

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