Dengue virus infection rate in field populations of female Aedes aegypti and Aedes albopictus in Singapore

被引：38

作者：

Chung, YK ^{[1
]}

Pang, FY ^{[1
]}

机构：

[1] Minist Environm, Vector Control & Res Dept, Singapore 228231, Singapore

来源：

TROPICAL MEDICINE & INTERNATIONAL HEALTH | 2002年 / 7卷 / 04期

关键词：

polymerase chain reaction; Aedes aegypti; Aedes albopictus; dengue virus; dengue serotypes;

D O I：

10.1046/j.1365-3156.2002.00873.x

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We developed a single-step reverse transcription-polymerase chain reaction (RT-PCR) followed by a seminested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein gene (NS3) of dengue viruses to type dengue viruses in field populations of female Aedes mosquitoes. This yielded diagnostic fragments of 169, 362, 265 and 426 base pairs for dengue virus types 1, 2, 3 and 4, respectively. From 1997 to 2000, 54 (6.9%) of 781 Aedes aegypti and 67 of 2256 Aedes albopictus screened were positive for dengue viruses, with a declining trend. The most common dengue virus type detected in the Aedes mosquitoes was dengue-1. Details on the change of one serotype to another in the mosquito population over three consecutive years are discussed.

引用

页码：322 / 330

页数：9

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