Purification and partial gene sequence of the tyrosine decarboxylase of Lactobacillus brevis IOEB 9809

被引：53

作者：

Lucas, P ^{[1
]}

Lonvaud-Funel, A ^{[1
]}

机构：

[1] Univ Bordeaux 2, Unite Associee INRA, Fac Oenol, Lab Biotechnol & Microbiol Appl, F-33405 Talence, France

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 211卷 / 01期

关键词：

tyrosine decarboxylase; tyramine; lactic acid bacterium; protein purification; gene sequencing; Lactobacillus brevis;

D O I：

10.1111/j.1574-6968.2002.tb11207.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Some lactic acid bacteria contain a tyrosine decarboxylase (TDC) which converts tyrosine to tyramine, a biogenic amine frequently encountered in fermented food and wine. Purification and microsequencing of the TDC of Lactobacillus brevis IOEB 9809 allowed us to determine a partial sequence of the TDC gene encoding 264 amino acids of the enzyme. Analysis of this protein sequence revealed typical features of pyridoxal phosphate-dependent amino acid decarboxylases while not any known decarboxylase was closely related to the TDC of L. brevis IOEB 9809. In addition, we could detect other L. brevis strains carrying a TDC gene in a rapid assay based oil the polymerase chain reaction. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：85 / 89

页数：5

共 17 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] PURIFICATION AND CHARACTERIZATION OF TYROSINE DECARBOXYLASE AND AROMATIC-L-AMINO-ACID DECARBOXYLASE [J].

BORRESEN, T ;

KLAUSEN, NK ;

LARSEN, LM ;

SORENSEN, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 993 (01) :108-115

[3] Improved screening procedure for biogenic amine production by lactic acid bacteria [J].

Bover-Cid, S ;

Holzapfel, WH .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 53 (01) :33-41

[4]

Coton E, 1998, J APPL MICROBIOL, V84, P143, DOI 10.1046/j.1365-2672.1998.00271.x

[5]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[6] BIOGENIC-AMINES AND THEIR PRODUCTION BY MICROORGANISMS IN FOOD [J].

HALASZ, A ;

BARATH, A ;

SIMONSARKADI, L ;

HOLZAPFEL, W .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1994, 5 (02) :42-49

[7]

Kaneko T, 2000, DNA RES, V7, P331, DOI 10.1093/dnares/7.6.331

[8]

LEJEUNE C, 1995, J APPL BACTERIOL, V78, P316

[9]

LIVINGSTONE CD, 1993, COMPUT APPL BIOSCI, V9, P745

[10]

Lonvaud-Funel A, 2001, FEMS MICROBIOL LETT, V199, P9, DOI 10.1016/S0378-1097(01)00157-4

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