Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn's disease

被引：93

作者：

Diederen, Kay ^{[1
,2
,3
]}

Li, Jia, V ^{[4
]}

Donachie, Gillian E. ^{[6
]}

de Meij, Tim G. ^{[1
,2
]}

de Waart, Dirk R. ^{[3
]}

Hakvoort, Theodorus B. M. ^{[3
]}

Kindermann, Angelika ^{[1
,2
]}

Wagner, Josef ^{[7
]}

Auyeung, Victoria ^{[4
,5
]}

te Velde, Anje A. ^{[3
]}

Heinsbroek, Sigrid E. M. ^{[3
]}

Benninga, Marc A. ^{[1
,2
]}

Kinross, James ^{[4
,5
]}

Walker, Alan W. ^{[6
]}

de Jonge, Wouter J. ^{[3
]}

Seppen, Jurgen ^{[3
]}

机构：

[1] Locat AMC, Amsterdam UMC, Dept Pediat Gastroenterol & Nutr, Amsterdam, Netherlands

[2] Vrije Univ Amsterdam Med Ctr, Amsterdam, Netherlands

[3] Locat AMC, Amsterdam UMC, Tytgat Inst Liver & Intestinal Res, Meibergdreef 69, NL-1105 BK Amsterdam, Netherlands

[4] Imperial Coll London, Div Digest Dis, Dept Metab Digest & Reprod, London, England

[5] Imperial Coll London, Dept Surg & Canc, London, England

[6] Univ Aberdeen, Rowett Inst, Aberdeen, Scotland

[7] Wellcome Sanger Inst, Pathogen Genom Grp, Hinxton, Cambs, England

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

基金：

欧盟地平线“2020”; 英国惠康基金; 欧洲研究理事会;

关键词：

INFLAMMATORY-BOWEL-DISEASE; DEXTRAN SULFATE SODIUM; INTESTINAL MICROBIOTA; NMR-SPECTROSCOPY; L-CARNITINE; RECEPTOR; COLITIS; TGR5; CORTICOSTEROIDS; IDENTIFICATION;

D O I：

10.1038/s41598-020-75306-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

A nutritional intervention, exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn's disease (CD). We characterized changes in the fecal microbiota and metabolome to identify the mechanism of EEN. Feces of 43 children were collected prior, during and after EEN. Microbiota and metabolites were analyzed by 16S rRNA gene amplicon sequencing and NMR. Selected metabolites were evaluated in relevant model systems. Microbiota and metabolome of patients with CD and controls were different at all time points. Amino acids, primary bile salts, trimethylamine and cadaverine were elevated in patients with CD. Microbiota and metabolome differed between responders and non-responders prior to EEN. EEN decreased microbiota diversity and reduced amino acids, trimethylamine and cadaverine towards control levels. Patients with CD had reduced microbial metabolism of bile acids that partially normalized during EEN. Trimethylamine and cadaverine inhibited intestinal cell growth. TMA and cadaverine inhibited LPS-stimulated TNF-alpha and IL-6 secretion by primary human monocytes. A diet rich in free amino acids worsened inflammation in the DSS model of intestinal inflammation. Trimethylamine, cadaverine, bile salts and amino acids could play a role in the mechanism by which EEN induces remission. Prior to EEN, microbiota and metabolome are different between responders and non-responders.

引用

页数：17

共 82 条

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