Glycomic profiling of cells and tissues by mass spectrometry: Fingerprinting and sequencing methodologies

被引:129
作者
Jang-Lee, Jihye [1 ]
North, Simon J.
Sutton-Smith, Mark
Goldberg, David
Panico, Maria
Morris, Howard
Haslam, Stuart
Dell, Anne
机构
[1] Imperial Coll London, Dept Mol Biosci, London, England
[2] Scripps PARC Inst, Adv Biomed Sci, Palo Alto, CA USA
来源
GLYCOBIOLOGY | 2006年 / 415卷
关键词
D O I
10.1016/S0076-6879(06)15005-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Over the past decade, rapid, high-sensitivity mass spectrometric strategies have been developed and optimized for screening for the types of N- and O-glycans present in a diverse range of biological material, including secretions, cell lines, tissues, and organs. These glycomic strategies are based on matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass fingerprinting of permethylated derivatives, combined with electrospray (ES) or MALDI tandem mass spectrometry (MS/MS) sequencing and gas chromatography (GC)-MS linkage analysis, complemented by chemical and enzymatic degradations. Protocols for these methods are described in the first part of this chapter. Glycomic experiments yield large volumes of MS data, and interpretation of the resulting spectra remains a time-consuming bottleneck in the process. In the second part of this chapter, we describe the use and operation of a mass spectral viewer program capable of displaying and automatically labeling spectra arising from MALDI fingerprinting of N-glycans.
引用
收藏
页码:59 / 86
页数:28
相关论文
共 33 条
  • [31] TANG H, 2005, 13 INT C INT SYST MO
  • [32] Electrospray ionization-ion trap mass spectrometry for structural analysis of complex N-linked glycoprotein oligosaccharides
    Weiskopf, AS
    Vouros, P
    Harvey, DJ
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (20) : 4441 - 4447
  • [33] Characterization of the oligosaccharides associated with the human ovarian tumor marker CA125
    Wong, NK
    Easton, RL
    Panico, M
    Sutton-Smith, M
    Morrison, JC
    Lattanzio, FA
    Morris, HR
    Clark, GF
    Dell, A
    Patankar, MS
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (31) : 28619 - 28634