Synthetic peptide combinatorial libraries: A method for the identification of bioactive peptides against phytopathogenic fungi

被引：45

作者：

Reed, JD

Edwards, DL

Gonzalez, CF

机构：

[1] TEXAS A&M UNIV,DEPT PLANT PATHOL & MICROBIOL,COLLEGE STN,TX 77843

[2] CERES TECHNOL INC,SAN ANTONIO,TX 78230

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1997年 / 10卷 / 05期

关键词：

antifungal; synthetic peptide;

D O I：

10.1094/MPMI.1997.10.5.537

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthetic combinatorial libraries were evaluated with an iterative process to identify a hexapeptide with broad-spectrum activity against selected phytopathogenic fungi. A D-amino acid hexapeptide (FRLKFH) and pentapeptide (FRLHF) exhibited activity against Fusarium oxysporum f. sp, lycopersici, Rhizoctonia solani (anastomosis group 1), Ceratocystis fagacearum, and Pythium ultimum, The peptides showed no hemolytic or mutagenic activity, Fluorescent microscopy studies with a membrane impermeant dye indicated that fungal cytoplasmic membranes were compromised rapidly and that the nuclear membrane was also affected.

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页码：537 / 549

页数：13

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