Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in cultured tobacco cells

被引：35

作者：

Yano, K

Matsui, S

Tsuchiya, T

Maeshima, M

Kutsuna, N

Hasezawa, S

Moriyasu, Y ^{[1
]}

机构：

[1] Univ Shizuoka, Sch Food & Nutr Sci, Shizuoka, Japan

[2] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi, Japan

[3] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrat Biosci, Kashiwa, Chiba, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2004年 / 45卷 / 07期

关键词：

autolysosome; autophagy; central vacuole; endocytosis; FM4-64; green fluorescent protein;

D O I：

10.1093/pcp/pch105

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent dyes and green fluorescent protein (GFP). Observation using the endocytosis markers, FM4-64 and Lucifer Yellow CH, suggested that there is a membrane flow from the plasma membrane to autolysosomes. Using these dyes as well as GFP-AtVam3p, sporamin-GFP and gamma-VM23-GFP fusion proteins as markers of the central vacuole, we found transport of components of the central vacuole to autolysosomes. Thus endocytosis and the supply from the central vacuole may contribute to the formation of autolysosomes.

引用

页码：951 / 957

页数：7