Lipase-catalyzed synthesis of tricaprylin in a medium solely composed of substrates. Water production and elimination

被引：26

作者：

Selmi, B ^{[1
]}

Gontier, E ^{[1
]}

Ergan, F ^{[1
]}

Barbotin, JN ^{[1
]}

Thomas, D ^{[1
]}

机构：

[1] FAC SCI,LAB GENIE CELLULAIRE,AMIENS,FRANCE

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1997年 / 20卷 / 05期

关键词：

esterification; immobilized lipase; tricaprylin synthesis; glycerides; water production and elimination; water activity;

D O I：

10.1016/S0141-0229(96)00132-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The conditions for tricaprylin synthesis using Lipozyme (immobilized 1,3-specific Mucor miehei lipase) in a medium solely composed of glycerol and caprylic acid (octanoic acid) in stoichiometric amounts are 60 degrees C with air ventilation in an open batch. The kinetics of mono-, di-, and trioctanoylglycerol synthesis and caprylic acid esterification are correlated with those corresponding to water production and water activity alone the esterification reaction, The technical feasibility of studying the time course of the reaction and water loss monitoring only by weight and Karl-Fisher measurements is also described. (C) 1997 by Elsevier Science Inc.

引用

页码：322 / 325

页数：4

共 17 条

[1] ENZYMATIC ESTERIFICATION OF GLYCEROL .1. LIPASE-CATALYZED SYNTHESIS OF REGIOISOMERICALLY PURE 1,3-SN-DIACYLGLYCEROLS [J].