Structural genomics reveals EVE as a new ASCH/PUA-related domain

被引：31

作者：

Bertonati, Claudia ^{[1
,2
,3
,4
]}

Punta, Marco ^{[1
,2
,3
]}

Fischer, Markus ^{[1
,2
,3
]}

Yachdav, Guy ^{[1
,2
,3
]}

Forouhar, Farhad ^{[5
]}

Zhou, Weihong ^{[5
]}

Kuzin, Alexander P. ^{[5
]}

Seetharaman, Jayaraman ^{[5
]}

Abashidze, Mariam ^{[5
]}

Ramelot, Theresa A. ^{[6
,7
]}

Kennedy, Michael A. ^{[6
,7
]}

Cort, John R. ^{[8
,9
,10
]}

Belachew, Adam ^{[11
]}

Hunt, John F. ^{[5
]}

Tong, Liang ^{[5
]}

Montelione, Gaetano T. ^{[3
,12
,13
,14
]}

Rost, Burkhard ^{[1
,2
,3
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[2] Columbia Univ, Ctr Computat Biol & Bioinformat C2B2, New York, NY 10032 USA

[3] Columbia Univ, NE Struct Genom Consortium NESG, New York, NY 10032 USA

[4] Univ Roma La Sapienza, Dept Biochem A Rossi Fanelli, I-00185 Rome, Italy

[5] Columbia Univ, Dept Biol Sci, NE Struct Genom Consortium, New York, NY 10027 USA

[6] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA

[7] Miami Univ, NE Struct Genom Consortium NESG, Oxford, OH 45056 USA

[8] Washington State Univ, Dept Chem, Richland, WA 99354 USA

[9] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

[10] Pacific NW Natl Lab, NE Struct Genom Consortium, Richland, WA 99352 USA

[11] Weill Cornell Med Coll, Dept Pharmacol, New York, NY 10021 USA

[12] Rutgers State Univ, Ctr Adv Biotechnol, Dept Mol Biol & Biochem, Piscataway, NJ 08854 USA

[13] NE Struct Genom Consortium, Piscataway, NJ 08854 USA

[14] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem, Piscataway, NJ 08854 USA

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2009年 / 75卷 / 03期

基金：

美国国家卫生研究院;

关键词：

structural genomics; protein function prediction; PUA domain-like domains; X-ray crystallography; NMR; CRYSTAL-STRUCTURE; BINDING DOMAINS; TRANSFER-RNA; PUA DOMAIN; ALIGNMENT; VISUALIZATION; PROTEINS; DNA; RECOGNITION; SEQUENCES;

D O I：

10.1002/prot.22287

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on several proteins recently solved by structural genomics consortia, in particular by the Northeast Structural Genomics consortium (NESG). The proteins considered in this study differ substantially in their sequences but they share a similar structural core, characterized by a pseudobarrel five-stranded beta sheet. This core corresponds to the PUA domain-like architecture in the SCOP database. By connecting sequence information with structural knowledge, we characterize a new subgroup of these proteins that we propose to be distinctly different from previously described PUA domain-like domains such as PUA proper or ASCH. We refer to these newly defined domains as EVE. Although EVE may have retained the ability of PUA domains to bind RNA, the available experimental and computational data suggests that both the details of its molecular function and its cellular function differ from those of other PUA domain-like domains. This study of EVE and its relatives illustrates how the combination of structure and genomics creates new insights by connecting a cornucopia of structures that map to the same evolutionary potential. Primary sequence information alone would have not been sufficient to reveal these evolutionary links.

引用

页码：760 / 773

页数：14

共 60 条

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