E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells

被引：66

作者：

Tilghman, RW ^{[1
]}

Hoover, RL ^{[1
]}

机构：

[1] Vanderbilt Univ, Sch Med, Dept Pathol, Med Ctr N U4202, Nashville, TN 37232 USA

来源：

FEBS LETTERS | 2002年 / 525卷 / 1-3期

关键词：

endothelial cell; lipid raft; tyrosine kinase; adhesion;

D O I：

10.1016/S0014-5793(02)03070-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we present data supporting the role of lipid rafts in endothelial cells during leukocyte adhesion. Following adhesion of THP-1 cells or antibody-mediated clustering, both E-selectin and intercellular adhesion molecule-1 (ICAM-1) partitioned into the detergent-insoluble portion of the endothelial cellular lysate. Sucrose gradient centrifugation revealed the partitioning of clustered E-selectin and ICAM-1 with the low-density fraction where they co-fractionated with src family kinases, markers of lipid rafts. Depleting the plasma membrane of cholesterol inhibited clustering of adhesion molecules following their antibody-induced crosslinking and inhibited their association with src kinases. Thus, our data suggest that E-selectin and ICAM-I associate with lipid rafts in human endothelial cells following leukocyte adhesion. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：83 / 87

页数：5