Fully automated, sequential tilt-series acquisition with Leginon

被引：140

作者：

Suloway, Christian ^{[2
]}

Shi, Jian ^{[2
]}

Cheng, Anchi ^{[3
]}

Pulokas, James ^{[3
]}

Carragher, Bridget ^{[3
]}

Potter, Clinton S. ^{[3
]}

Zheng, Shawn Q. ^{[4
,5
]}

Agard, David A. ^{[4
,5
]}

Jensen, Grant J. ^{[1
,2
]}

机构：

[1] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

[3] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[4] Univ Calif San Francisco, WM Keck Adv Microscopy Lab, San Francisco, CA 94158 USA

[5] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2009年 / 167卷 / 01期

基金：

美国国家卫生研究院;

关键词：

TEM; Cryo-electron microscopy; Electron microscopy; Automation; Tomography; ELECTRON TOMOGRAPHY; DATA-COLLECTION; MICROSCOPY; RECONSTRUCTION; MICROGRAPHS; CRYOTOMOGRAPHY; SYSTEM; CELLS;

D O I：

10.1016/j.jsb.2009.03.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron tomography has become a uniquely powerful tool for investigating the structures of individual cells, viruses, and macromolecules. Data collection is, however, time consuming and requires expensive instruments. To optimize productivity, we have incorporated one of the existing tilt-series acquisition programs, UCSF Tomo, into the well-developed automatic electron microscopy data collection package Leginon to enable fully automatic, sequential tilt-series acquisition. Here we describe how UCSF Tomo was integrated into Leginon, what users must do to set up a data collection session, how the automatic collection proceeds, how archived data about the process can be accessed and used, and how the software has been tested. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：11 / 18

页数：8

共 25 条

[1] Location and architecture of the Caulobacter crescentus chemoreceptor array [J].