Photoresponsive nanogels formed by the self-assembly of spiropyrane-bearing pullulan that act as artificial molecular chaperones

被引：104

作者：

Hirakura, T

Nomura, Y

Aoyama, Y

Akiyoshi, K

机构：

[1] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[2] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Sakyo Ku, Kyoto 6068501, Japan

[3] Tokyo Med & Dent Univ, Ctr Excellence, Program Frontier Res Mol Destruct & Reconstruct T, Tokyo 1010062, Japan

来源：

BIOMACROMOLECULES | 2004年 / 5卷 / 05期

关键词：

D O I：

10.1021/bm049860o

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel photoresponsive nanogels were prepared by the self-assembly of spiropyrane-bearing pullulan (SpP). The solution properties of the nanogels could be controlled by photostimulation via isomerization between hydrophobic spiropyrane and hydrophilic merocyanine. The molecular chaperone-like activity of the nanogels in protein refolding was investigated. The activity of citrate synthase significantly increased when the amphiphilicity of SpP nanogels was switched by photostimulation.

引用

收藏

页码：1804 / 1809

页数：6

相关论文

共 36 条

[1] Molecular chaperone-like activity of hydrogel nanoparticles of hydrophobized pullulan: Thermal stabilization with refolding of carbonic anhydrase B [J].

Akiyoshi, K ;

Sasaki, Y ;

Sunamoto, J .

BIOCONJUGATE CHEMISTRY, 1999, 10 (03) :321-324

[2] Microscopic structure and thermoresponsiveness of a hydrogel nanoparticle by self-assembly of a hydrophobized polysaccharide [J].

Akiyoshi, K ;

Deguchi, S ;

Tajima, H ;

Nishikawa, T ;

Sunamoto, J .

MACROMOLECULES, 1997, 30 (04) :857-861

[3] SELF-AGGREGATES OF HYDROPHOBIZED POLYSACCHARIDES IN WATER - FORMATION AND CHARACTERISTICS OF NANOPARTICLES [J].

AKIYOSHI, K ;

DEGUCHI, S ;

MORIGUCHI, N ;

YAMAGUCHI, S ;

SUNAMOTO, J .

MACROMOLECULES, 1993, 26 (12) :3062-3068

[4] Controlled association of amphiphilic polymers in water:: Thermosensitive nanoparticles formed by self-assembly of hydrophobically modified pullulans and poly(N-isopropylacrylamides) [J].

Akiyoshi, K ;

Kang, EC ;

Kurumada, S ;

Sunamoto, J ;

Principi, T ;

Winnik, FM .

MACROMOLECULES, 2000, 33 (09) :3244-3249

[5] Photoswitchable molecular receptors [J].

Alfimov, MV ;

Fedorova, OA ;

Gromov, SP .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2003, 158 (2-3) :183-198

[6] Spiropyran derivative of an elastin-like bioelastic polymer:: Photoresponsive molecular machine to convert sunlight into mechanical work [J].

Alonso, M ;

Reboto, V ;

Guiscardo, L ;

San Martín, A ;

Rodríguez-Cabello, JC .

MACROMOLECULES, 2000, 33 (26) :9480-9482

[7] RHEOLOGY OF SMALL SPHERICAL POLYSTYRENE MICROGELS - A DIRECT PROOF FOR A NEW TRANSPORT MECHANISM IN BULK POLYMERS BESIDES REPTATION [J].

ANTONIETTI, M ;

PAKULA, T ;

BREMSER, W .

MACROMOLECULES, 1995, 28 (12) :4227-4233

[8] Molecular imprinting within hydrogels [J].

Byrne, ME ;

Park, K ;

Peppas, NA .

ADVANCED DRUG DELIVERY REVIEWS, 2002, 54 (01) :149-161

[9]

CLELAND JL, 1992, J BIOL CHEM, V267, P13327

[10]

Gan DJ, 2001, J AM CHEM SOC, V123, P7511, DOI 10.1021/ja010609f

← 1 2 3 4 →