An integrin-targeted non-viral vector for pulmonary gene therapy

被引：73

作者：

Jenkins, RG

Herrick, SE

Meng, QH

Kinnon, C

Laurent, GJ

McAnulty, RJ

Hart, SL

机构：

[1] UCL, Royal Free & Univ Coll Med Sch, Ctr Cardiopulm Biochem & Resp Med, Rayne Inst, London WC1E 6JJ, England

[2] UCL, Inst Child Hlth, Mol Immunol Unit, London, England

来源：

GENE THERAPY | 2000年 / 7卷 / 05期

基金：

英国惠康基金;

关键词：

integrin-targeting vector; gene therapy; lung;

D O I：

10.1038/sj.gt.3301095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene therapy offers potential for the treatment of severe respiratory diseases. However, the vectors which are currently available have drawbacks limiting their therapeutic application. Here we report on an integrin-targeted, non-viral gene delivery system for pulmonary gene transfer. We demonstrate that this vector can deliver the lacZ reporter gene to the lung, transfecting bronchial epithelium and parenchymal cells with similar efficiency to an adenoviral Vector and with greater efficiency than a cationic liposome. In addition, vector administration can be repeated leading to further gene expression without inducing inflammation. The advantages of this novel gene delivery system provide considerable potential for targeted gene therapy in vivo.

引用

页码：393 / 400

页数：8

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