Mutation of the RIIβ subunit of protein kinase A differentially affects lipolysis but not gene induction in white adipose tissue

被引:55
作者
Planas, JV [1 ]
Cummings, DE [1 ]
Idzerda, RL [1 ]
McKnight, GS [1 ]
机构
[1] Univ Washington, Dept Pharmacol, Sch Med, Seattle, WA 98195 USA
关键词
D O I
10.1074/jbc.274.51.36281
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Targeted disruption of the RII beta subunit of protein kinase A (PKA) produces lean mice that resist diet-induced obesity. In this report we examine the effects of the RII beta knockout on white adipose tissue physiology, Loss of RII beta is compensated by an increase in the RI alpha isoform, generating an isoform switch from a type II to a type I PKA, Type I holoenzyme binds cAMP more avidly and is more easily activated than the type II enzyme. These alterations are associated with increases in both basal kinase activity and the basal rate of lipolysis, possibly contributing to the lean phenotype, However, the ability of both beta(3)-selective and nonspecific beta-adrenergic agonists to stimulate lipolysis is markedly compromised in mutant white adipose tissue. This defect was found in vitro and in vivo and does not result from reduced expression of beta-adrenergic receptor or hormone-sensitive lipase genes. In contrast, beta-adrenergic stimulated gene transcription remains intact, and the expression of key genes involved in lipid metabolism is normal under both fasted and fed conditions. We suggest that the R subunit isoform switch disrupts the subcellular localization of PHA that is required for efficient transduction of signals that modulate lipolysis but not for those that mediate gene expression.
引用
收藏
页码:36281 / 36287
页数:7
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