Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages

被引：61

作者：

Rosas-Magallanes, Vania

Stadthagen-Gomez, Gustavo

Rauzier, Jean

Barreiro, Luis B.

Tailleux, Ludovic

Boudou, Frederic

Griffin, Ruth

Nigou, Jerome

Jackson, Mary

Gicquel, Brigitte

Neyrolles, Olivier

机构：

[1] Inst Pasteur, Unit Mycobacterial Genet, F-75724 Paris 15, France

[2] Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, London SW7 2AZ, England

[3] CNRS, UMR 5089, Inst Pharmacol & Biol Struct, Dept Mol Mechanisms Mycobacterial Infect, Toulouse, France

[4] CNRS, URA 2172, Paris, France

来源：

INFECTION AND IMMUNITY | 2007年 / 75卷 / 01期

关键词：

D O I：

10.1128/IAI.00058-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

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页码：504 / 507

页数：4

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