Methylation analysis of the imprinted DLK1-GTL2 domain supports the random parental origin of the IGH-involving del(14q) in B-cell malignancies

被引：6

作者：

Katrincsakova, Beata ^{[1
,2
,3
,4
]}

Takeda, Haruko ^{[3
,4
]}

Urbankova, Helena ^{[1
,2
]}

Michaux, Lucienne ^{[1
]}

Jarosova, Marie ^{[2
]}

Vandenberghe, Peter ^{[1
]}

Georges, Michel ^{[3
,4
]}

Charlier, Carole ^{[3
,4
]}

Wlodarska, Iwona ^{[1
]}

机构：

[1] Catholic Univ Louvain, Ctr Human Genet, B-3000 Louvain, Belgium

[2] Palacky Univ Olomouc, Dept Hematooncol, Olomouc, Czech Republic

[3] Univ Liege, Unit Anim Genom, GIGA R, B-4000 Liege, Belgium

[4] Univ Liege, Dept Anim Sci, Fac Vet Med, B-4000 Liege, Belgium

来源：

EPIGENETICS | 2009年 / 4卷 / 07期

关键词：

deletion; immunoglobulin heavy chain locus; tumor suppressor gene; microRNA; DLK1/GTL2; imprinted genes; CHRONIC LYMPHOCYTIC-LEUKEMIA; DOWN-REGULATION; WILMS-TUMOR; EXPRESSION; CANCER; GENE; PROGRESSION; ACTIVATION; MICRORNAS; CLUSTER;

D O I：

10.4161/epi.4.7.9924

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leukemias/lymphomas with IGH-involving del(14q)(1) commonly lose the DLK1-GTL2 imprinted domain that comprises several paternally and maternally expressed genes, including a cluster of microRNAs. Given that deletion of this region could lead to inactivation of a monoallelically expressed tumor suppressor gene, our study aimed at determination of the parental origin of del(14q/IGH). The designed allele-specific methylation study of the DLK1/GTL2 intergenic differentially methylated region allowed us to determine the parental origin of del(14q/IGH) in 9/20 analyzed cases. In six cases del(14q/IGH) was of the paternal origin and in three cases of the maternal origin. These findings argue against the concept that a TSG/anti-oncomir located in the imprinted region is systematically inactivated by a targeted deletion of its functional allele.

引用

页码：469 / 475

页数：7

共 25 条

[1] Cumulative dosage effect of a RAD51L1/HMGA2 fusion and RAD51L1 loss in a case of Pseudo-Meigs' syndrome [J].

Amant, F ;

Debiec-Rychter, M ;

Schoenmakers, EFPM ;

Hagemeijer-Hausman, A ;

Vergote, I .

GENES CHROMOSOMES & CANCER, 2001, 32 (04) :324-329

[2] Epigenetic alteration at the DLK1-GTL2 imprinted domain in human neoplasia:: analysis of neuroblastoma, phaeochromocytoma and Wilms' tumour [J].

Astuti, D ;

Latif, F ;

Wagner, K ;

Gentle, D ;

Cooper, WN ;

Catchpoole, D ;

Grundy, R ;

Ferguson-Smith, AC ;

Maher, ER .

BRITISH JOURNAL OF CANCER, 2005, 92 (08) :1574-1580

[3] Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia [J].

Calin, GA ;

Dumitru, CD ;

Shimizu, M ;

Bichi, R ;

Zupo, S ;

Noch, E ;

Aldler, H ;

Rattan, S ;

Keating, M ;

Rai, K ;

Rassenti, L ;

Kipps, T ;

Negrini, M ;

Bullrich, F ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15524-15529

[4] A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia [J].

Calin, GA ;

Ferracin, M ;

Cimmino, A ;

Di Leva, G ;

Shimizu, M ;

Wojcik, SE ;

Iorio, MV ;

Visone, R ;

Sever, NI ;

Fabbri, M ;

Iuliano, R ;

Palumbo, T ;

Pichiorri, F ;

Roldo, C ;

Garzon, R ;

Sevignani, C ;

Rassenti, L ;

Alder, H ;

Volinia, S ;

Liu, CG ;

Kipps, TJ ;

Negrini, M ;

Croce, CM .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (17) :1793-1801

[5] MicroRNAs and cancer [J].

Dalmay, T. .

JOURNAL OF INTERNAL MEDICINE, 2008, 263 (04) :366-375

[6] MicroRNAs and noncoding RNAs in hematological malignancies: molecular, clinical and therapeutic implications [J].

Fabbri, M. ;

Garzon, R. ;

Andreeff, M. ;

Kantarjian, H. M. ;

Garcia-Manero, G. ;

Calin, G. A. .

LEUKEMIA, 2008, 22 (06) :1095-1105

[7] Survey of allelic expression using EST mining [J].

Ge, B ;

Gurd, S ;

Gaudin, T ;

Dore, C ;

Lepage, P ;

Harmsen, E ;

Hudson, TJ ;

Pastinen, T .

GENOME RESEARCH, 2005, 15 (11) :1584-1591

[8] Selective loss of MEG3 expression and intergenic differentially methylated region hypermethylation in the MEG3/DLK1 locus in human clinically nonfunctioning pituitary adenomas [J].

Gejman, Roger ;

Batista, Dalia L. ;

Zhong, Ying ;

Zhou, Yunli ;

Zhang, Xun ;

Swearingen, Brooke ;

Stratakis, Constantine A. ;

Hedley-Whyte, E. Tessa ;

Klibanski, Anne .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2008, 93 (10) :4119-4125

[9] Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma [J].

Kawakami, T ;

Chano, T ;

Minami, K ;

Okabe, H ;

Okada, Y ;

Okamoto, K .

HUMAN MOLECULAR GENETICS, 2006, 15 (06) :821-830

[10] Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12 [J].

Lin, SP ;

Youngson, N ;

Takada, S ;

Seitz, H ;

Reik, W ;

Paulsen, M ;

Cavaille, J ;

Ferguson-Smith, ACF .

NATURE GENETICS, 2003, 35 (01) :97-102

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