Cell-type-specific interactions at regulatory motifs in the first intron of the lamin A gene

被引:8
作者
Arora, P [1 ]
Muralikrishna, B [1 ]
Parnaik, VK [1 ]
机构
[1] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India
来源
FEBS LETTERS | 2004年 / 568卷 / 1-3期
关键词
nuclear lamina; lamin A promoter; A-type lamin; lamin C2; intronic regulatory element; gene expression;
D O I
10.1016/j.febslet.2004.05.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lamins A, C and C2 are alternatively spliced products of the LMNA gene; lamins A and C are expressed in differentiated somatic cells, whereas lamin C2 is expressed in germ cells. We have analyzed a segment of the first intron of the LMNA gene for cell-type-specific regulatory elements. We identified a 420-bp fragment that increased promoter activity in lamin A-expressing cells but repressed activity in undifferentiated cells. DNase I footprinting and electrophoretic mobility shift assays revealed two binding motifs, footprinted region A (FPRA) and FPRB. The hepatocyte nuclear factor-3beta was bound to FPRA only in somatic cell extracts and this motif had an inhibitory effect on promoter activity. The retinoic X receptor beta, RXRbeta, bound near FPRB with extracts from lamin A- or C2-expressing cells, and this site enhanced promoter activity. We have, thus, identified two novel binding sites for transcription factors in a region likely to function as an important regulatory element for the cell-type-specific transcription of A-type lamins. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:122 / 128
页数:7
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