Microsphere resin chromatography combined with microbial biotransformation for the separation and purification of salvianolic acid B in aqueous extract of roots of Salvia multiorrihza Bunge

被引:25
作者
Kan, Shidong [2 ]
Li, Ji'an [1 ]
Huang, Weiyi [2 ]
Shao, Lei [1 ]
Chen, Daijie [1 ]
机构
[1] Shanghai Inst Pharmaceut Ind, Shanghai 200040, Peoples R China
[2] Nanjing Agr Univ, Life Sci Coll, Dept Microbiol, Nanjing 210095, Peoples R China
关键词
Microbial biotransformation; Microsphere resin; Salvianolic acid B; Rosmarinic acid; Fusarium graminearum; Carboxylesterase; COUNTER-CURRENT CHROMATOGRAPHY; MILTIORRHIZA BUNGE; TRICHOTHECENE BIOSYNTHESIS; DANSHEN; CELLS; RADIX;
D O I
10.1016/j.chroma.2009.02.084
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Salvianolic acid B was separated and purified from Salvia miltiorrhiza Bunge (danshen) by microbial transformation together with chromatography of microsphere resin. The aqueous extract of danshen was transformed by Fusarium graminearum in a bioreactor containing phosphate buffer (PBS), in which rosmarinic acid was transformed into danshensu and caffeic acid and the yield of salvianolic acid B was higher than 85%. After biotransformation, salvianolic acid B was purified by microsphere resin. A parallel test for making a comparison of microsphere resin chromatography between elution by methanol water solution and water was done. The purity of salvianolic acid B was up to 95% at the yield of 62% when impurities and salvianolic acid B were eluted by 45% and 55% methanol solution respectively. The purity of salvianolic acid B was up to 99% at the yield of 90% when distilled water was used to elute the impurities and salvianolic acid B. The total yield of salvianolic acid B was up to 75% at the purity over 99% while biotransformation combined with microsphere resin chromatography by water elution. Microbial biotransformation together with water elution of microsphere resin supplied an efficient method to eliminate the micromolecular impurities and a possible method to purify water-soluble compounds in traditional Chinese medicine. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:3881 / 3886
页数:6
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