Microstructured arrays with pre-synthesized capture probes for DNA detection based on metal nanoparticles and silver enhancement

被引：14

作者：

Zhang, GJ ^{[1
]}

Möller, R ^{[1
]}

Kretschmer, R ^{[1
]}

Csáki, A ^{[1
]}

Fritzsche, W ^{[1
]}

机构：

[1] Inst Phys High Technol, D-07702 Jena, Germany

来源：

JOURNAL OF FLUORESCENCE | 2004年 / 14卷 / 04期

关键词：

DNA microarray; gold nanoparticles; micro structures;

D O I：

10.1023/B:JOFL.0000031818.39925.4c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal.

引用

收藏

页码：369 / 375

页数：7

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