Analysis of phytochelatins in plant matrices by pre-column derivatization, high-performance liquid chromatography and fluorescence-detection

被引：27

作者：

Döring, S ^{[1
]}

Korhammer, S ^{[1
]}

Oetken, M ^{[1
]}

Markert, B ^{[1
]}

机构：

[1] Int Hsch Inst Zittau, Lehrstuhl Umweltverfahrenstechn, D-02763 Zittau, Germany

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 366卷 / 03期

关键词：

D O I：

10.1007/s002160050062

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive method for the determination of phytochelatins in plant matrices by pre-column derivatization with monobromobimane (mBrB) and high-performance liquid chromatography (HPLC) on reversed phases and fluorescence-detection has been developed and applied to cucumber sprouts (Cucumis sativus) treated with cadmium and to the water moss fontinalis antipyretica (Cd in environmentally-relevant concentrations). Whereas phytochelatins were found in the Cd-treated sprouts, no phytochelatins were detected in Fontinalis antipyretica.

引用

页码：316 / 318

页数：3

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