PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR

被引：505

作者：

Marshall, OJ ^{[1
]}

机构：

[1] Royal Childrens Hosp, Murdoch Childrens Res Inst, Parkville, Vic 3052, Australia

来源：

BIOINFORMATICS | 2004年 / 20卷 / 15期

关键词：

D O I：

10.1093/bioinformatics/bth254

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.

引用

页码：2471 / 2472

页数：2