Molecular vs. phenotypic sexing in Red Knots

被引:61
作者
Baker, AJ
Piersma, T [1 ]
机构
[1] Royal Ontario Museum, Ctr Biodivers & Conservat Biol, Toronto, ON M5S 2C6, Canada
[2] Univ Toronto, Dept Zool, Toronto, ON M5S 1A1, Canada
[3] Netherlands Inst Sea Res, NL-1790 AB Den Burg, Texel, Netherlands
[4] Univ Groningen, Ctr Ecol & Evolut Studies, NL-9750 AA Haren, Netherlands
[5] Univ Toronto, Dept Zool, Toronto, ON M5S 1A1, Canada
来源
CONDOR | 1999年 / 101卷 / 04期
关键词
Calidris canutus; CHD genes; molecular sexing; morphometrics; plumage; Red Knots; shorebirds;
D O I
10.2307/1370083
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Using a reference collection of blood samples of Red Knots (Calidris canutus) from Florida and Alaska whose sex was determined by dissection, we demonstrate that the molecular method of sexing based on the size of polymerase chain reaction (PCR) products from the CHD genes after digestion with Hae III accurately identifies gender in this species. In males, the 110 basepair PCR product is cleaved into two smaller fragments, whereas in females this fragment remains uncut. Molecular sexing of other samples of Red Knots from the Dutch Wadden Sea, southern Brazil, and Delaware Bay revealed that methods using a discriminant function on size or plumage dimorphism are only partly effective in correctly assigning sex of birds. Given the low cost and accuracy of molecular sexing on very small blood samples, we anticipate that it will find increasing use in studies of the evolutionary ecology of shorebirds and other avian species.
引用
收藏
页码:887 / 893
页数:7
相关论文
共 29 条
[1]  
Baker A.J., 1999, Wader Study Group Bulletin, V88, P64
[2]  
BARTER M, 1992, WADER STUDY GROUP S, V64, P64
[3]  
Davidson, 1992, WADER STUDY GROUP S, V64, P187
[4]  
Davis C J, 1992, Surg Laparosc Endosc, V2, P64
[5]   New tools for sex identification and the study of sex allocation in birds [J].
Ellegren, H ;
Sheldon, BC .
TRENDS IN ECOLOGY & EVOLUTION, 1997, 12 (07) :255-259
[6]   First gene on the avian W chromosome (CHD) provides a tag for universal sexing of non-ratite birds [J].
Ellegren, H .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1996, 263 (1377) :1635-1641
[7]   Sex identification in birds using two CHD genes [J].
Griffiths, R ;
Daan, S ;
Dijkstra, C .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1996, 263 (1374) :1251-1256
[8]   A CHD1 gene is Z chromosome linked in the chicken Gallus domesticus [J].
Griffiths, R ;
Korn, RM .
GENE, 1997, 197 (1-2) :225-229
[9]   Optimal map projections for analysing long-distance migration routes [J].
Gudmundsson, GA ;
Alerstam, T .
JOURNAL OF AVIAN BIOLOGY, 1998, 29 (04) :597-605
[10]  
Harrington B., 1996, FLIGHT RED KNOT