Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants

被引：11

作者：

Brewer, GJ

Jones, TT

Wallimann, T

Schlattner, U

机构：

[1] So Illinois Univ, Sch Med, Dept Med Microbiol & Immunol, Springfield, IL 62794 USA

[2] Swiss Fed Inst Technol, Inst Cell Biol, CH-8093 Zurich, Switzerland

来源：

MITOCHONDRION | 2004年 / 4卷 / 01期

基金：

美国国家卫生研究院;

关键词：

anti-oxidant; brain mitochondria; creatine kinase; respiration; ROS;

D O I：

10.1016/j.mito.2004.06.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA +/- SCAVEGR(TM) anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher V-max and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. (C) 2004 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：49 / 57

页数：9

共 40 条

[1] Glutathione depletion enforces the mitochondrial permeability transition and causes cell death in HL60 cells that overexpress Bcl-2 [J].