Imaging diacylglycerol dynamics at organelle membranes

被引：59

作者：

Sato, Moritoshi

Ueda, Yoshibumi

Umezawa, Yoshio ^{[1
]}

机构：

[1] Univ Tokyo, Dept Chem, Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama, Japan

来源：

NATURE METHODS | 2006年 / 3卷 / 10期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

D O I：

10.1038/nmeth930

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence imaging is a powerful technique to visualize spatiotemporal dynamics of biomolecules in living cells. We describe fluorescent indicators for a lipid second messenger, diacylglycerol (DAG), which allow the localized analysis of DAG dynamics at subcellular membranes. We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes.

引用

收藏

页码：797 / 799

页数：3

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