454 pyrosequencing reveals changes in the faecal microbiota of adults consuming Lactobacillus casei Zhang

被引：70

作者：

Zhang, Jiachao ^{[1
]}

Wang, Lifeng ^{[1
]}

Guo, Zhuang ^{[1
]}

Sun, Zhihong ^{[1
]}

Gesudu, Qimu ^{[1
]}

Kwok, Laiyu ^{[1
]}

Menghebilige ^{[1
]}

Zhang, Heping ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Educ Minist PR China, Key Lab Dairy Biotechnol & Bioengn, Dept Food Sci & Engn, Hohhot 010018, Peoples R China

来源：

FEMS MICROBIOLOGY ECOLOGY | 2014年 / 88卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Lactobacillus casei Zhang; intestinal microbiota; 454; pyrosequencing; INDUCED IMMUNE-RESPONSES; GUT MICROBIOTA; STRAIN SHIROTA; FERMENTED MILK; INNER-MONGOLIA; PROBIOTICS; HEALTH; BACTERIA; IDENTIFICATION; CONSUMPTION;

D O I：

10.1111/1574-6941.12328

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Probiotics are believed to help to maintain a healthy balance of the human gut microbiota. Lactobacillus casei Zhang (LcZ) is a novel potential probiotic isolated from the naturally fermented food koumiss. To better understand the impact of this potential probiotic on human intestinal microbiota, 24 subjects were randomly recruited for a longitudinal study: the subjects were required to consume LcZ for 28days, and faecal samples were collected prior to, during and after the LcZ consumption phase. Alterations in the gut microbiota were monitored using 454 pyrosequencing and quantitative polymerase chain reaction (q-PCR) technologies. We found that the consumption of LcZ significantly altered the composition of intestinal microbiota (P<0.001) and the gut microbiota diversity. Further analysis at the genus level revealed a positive correlation between LcZ and Prevotella, Lactobacillus, Faecalibacterium, Propionibacterium, Bifidobacterium and an unidentified genus from Bacteroidaceae and Lachnospiraceae and a negative correlation between LcZ administration and the presence of Clostridium, Phascolarctobacterium, Serratia, Enterococcus, Shigella and Shewanella. Furthermore, these changes were confirmed by q-PCR data.

引用

页码：612 / 622

页数：11

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