Identification and mapping of a T-DNA induced flower mutation in Arabidopsis thaliana

Collection of the T-DNA tagged lines of Arabidopsis thaliana have been created by Agrobacterium-mediated root transformation, Transgenic lines produced by this method have been screened for morphogenic mutations. A flower mutation with increased number of stamens and carpels (scaf1) was identified. This mutation has similar but weaker phenotype than the known mutant superman. Two mapping procedures, with visible and molecular markers, were used to locate scaf1 flower mutation, Genetic analysis showed that this mutation is located on chromosome 3 near gl1 gene. It is probably one of the SUPERMAN epigenetic alleles.