Nigerian rotavirus serotype GS could not be typed by PCR due to nucleotide mutation at the 3' end of the primer binding site

被引:75
作者
Adah, MI
Rohwedder, A
Olaleyle, OD
Werchau, H
机构
[1] RUHR UNIV BOCHUM,DEPT MED MICROBIOL & VIROL,D-44801 BOCHUM,GERMANY
[2] UNIV IBADAN,COLL MED,UNIV COLL HOSP,DEPT VIROL,IBADAN,NIGERIA
关键词
D O I
10.1007/s007050050206
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A rotavirus strain HMG89 from Nigeria with short electrophoretic pattern was typed G3 by PCR. A cDNA clone from the PCR product which hybridised in Northern blots to RNA segment 9 of the homologous Nigerian rotavirus strain HMG89 and laboratory reference strain 69M but not to other mammalian group A rotaviruses was sequenced. The VP7 gene 9 sequence is 1060 nucleotides long with two base deletions at positions 1034-1035. Sequence analysis of the primer (aAT8) used in the previous PCR serotyping assay revealed a mutation in one of the three nucleotide bases at the 3' end of the primer binding site accounting for our inability to serotype G8 strains in our samples. These findings demonstrate that PCR analysis can, albeit infrequently, lead to error in typing of rotaviruses due to small numbers of mutations in the primer binding region.
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收藏
页码:1881 / 1887
页数:7
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