Purification and characterization of broad bean lipoxygenase isoenzymes

被引：31

作者：

Clemente, A ^{[1
]}

Olías, R ^{[1
]}

Olías, JM ^{[1
]}

机构：

[1] Inst Grasa, Dept Fisiol & Tecnol Prod Vegetales, Seville 41012, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 04期

关键词：

broad bean; lipoxygenase; purification; isoenzymes; hydroperoxides; ketodienes;

D O I：

10.1021/jf990463s

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two lipoxygenase isoenzymes, BBL-1 and BBL-2, were purified from broad beans. Fractionation of globulins and albumins by ionic strength was preferred to the classical water extraction system and the ammonium sulfate fractionation as initial purification steps. From the albumin fraction, BBL-1 and BBL-2 were purified 17.6 and 35.7-fold, respectively, by conventional gel. filtration and ion-exchange chromatography. The molecular weight of both BBL-1. and BBL-2 was 97 kDa with a maximal activity around pH 5.8; however, they showed a significant difference in their K-m values for linoleic acid: 2.3 and 0.25 mM for BBL-1 and BBL-2, respectively. BBL-1 produced hydroperoxides and ketodienes while BBL-2 produced exclusively hydroperoxides.

引用

页码：1070 / 1075

页数：6

共 32 条

[1] LIPOXYGENASE ISOENZYMES OF FRENCH BEAN PERICARP - SEPARATION, CHARACTERIZATION AND CHLOROPHYLL-BLEACHING ACTIVITY [J].

ABBAS, J ;

ROUETMAYER, MA ;

LAURIERE, C .

PHYTOCHEMISTRY, 1989, 28 (04) :1019-1024

[2] PROPERTIES OF BROAD BEAN LIPOXYGENASE [J].

ALOBAIDY, HM ;

SIDDIQI, AM .

JOURNAL OF FOOD SCIENCE, 1981, 46 (02) :622-&

[3]

Axelrod B., 1981, METHODS ENZYMOLOGY, V71, P441, DOI DOI 10.1016/0076-6879(81)71055-3

[4]

BEAUX Y, 1974, ANN TECHNOL AGR, V23, P309

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] Protein quality of chickpea (Cicer arietinum L.) protein hydrolysates [J].

Clemente, A ;

Vioque, J ;

Sánchez-Vioque, R ;

Pedroche, J ;

Bautista, J ;

Millán, F .

FOOD CHEMISTRY, 1999, 67 (03) :269-274

[7]

COHEN JD, 1984, J CHROMATOGR, V313, P194

[8]

ENDRES JG, 1989, LEGUMES CHEM TECHNOL, P139

[9]

ESKIN NAM, 1974, PHYTOCHEMISTRY, P2713

[10] RECENT INVESTIGATIONS INTO THE LIPOXYGENASE PATHWAY OF PLANTS [J].

GARDNER, HW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1084 (03) :221-239

← 1 2 3 4 →