Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development

被引:150
作者
Ladher, RK
Church, VL
Allen, S
Robson, L
Abdelfattah, A
Brown, NA
Hattersley, G
Rosen, V
Luyten, FP
Dale, L
Francis-West, PH
机构
[1] Guys Hosp, Guys Kings & St Thomas Sch Dent, Dept Craniofacial Dev, London SE1 9RT, England
[2] Queen Mary Univ London, Dept Neurosci, London E1 4NS, England
[3] Univ London St Georges Hosp, Sch Med, Dept Anat & Dev Biol, London SW17 0RE, England
[4] Genet Inst Inc, Cambridge, MA 02140 USA
[5] UZ Leuven, Dept Rheumatol, B-3212 Pellenberg, Belgium
[6] UCL, Dept Anat & Dev Biol, London WC1E 6BT, England
基金
英国惠康基金;
关键词
Sfrp-2 (SDF-5); Frzb; Wnt antagonist; neural; limb; heart; craniofacial; placodes; myogenesis; chondrogenesis;
D O I
10.1006/dbio.1999.9586
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Wnt genes are known to play fundamental roles during patterning and development of a number of embryonic structures. Receptors for Wnts are members of the Frizzled family of proteins containing a cysteine-rich domain (CRD) that binds the Wnt protein. Recently several secreted frizzled-related proteins (Sfrps) that also contain a CRD have been identified and some of these can both bind and antagonise Wnt proteins. In this paper we report the expression patterns of the chick homologues of Frzb, a known Wnt antagonist, and Sfrp-2. Both genes are expressed in areas where Wnts are known to play a role in development, including the neural tube, myotome, cartilage, and sites of epithelial-mesenchymal interactions. Initially, Sfrp-2 and Frzb are expressed in overlapping areas in the neural plate and neural tube, whereas later, they have distinct patterns. In particular Sfrp-2 is associated with myogenesis while Frzb is associated with chondrogenesis, suggesting that they play different roles during development. Finally, we have used the early Xenopus embryo as an in vivo assay to show that Sfrp-2, like Frzb, is a Wnt antagonist. These results suggest that Sfrp-2 and Frzb may function in the developing embryo by modulating Wnt signalling. (C) 2000 Academic Press.
引用
收藏
页码:183 / 198
页数:16
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