Highly Pathogenic Avian Influenza Virus Subtype H5N1 in Africa: A Comprehensive Phylogenetic Analysis and Molecular Characterization of Isolates

被引:86
作者
Cattoli, Giovanni [1 ]
Monne, Isabella [1 ]
Fusaro, Alice [1 ]
Joannis, Tony M. [2 ]
Lombin, Lami H. [2 ]
Aly, Mona M. [3 ]
Arafa, Abdel S. [3 ]
Sturm-Ramirez, Katharine M. [4 ]
Couacy-Hymann, Emmanuel [5 ]
Awuni, Joseph A. [6 ]
Batawui, Komla B. [7 ]
Awoume, Kodzo A. [7 ]
Aplogan, Gilbert L. [8 ]
Sow, Adama [9 ]
Ngangnou, Andre C. [10 ]
Hamza, Iman M. El Nasri [11 ]
Gamatie, Djibo [12 ]
Dauphin, Gwenaelle [13 ]
Domenech, Joseph M. [13 ]
Capua, Ilaria [1 ]
机构
[1] Ist Zooprofilatt Sperle Venezie, OIE FAO Reference Lab Avian Influenza & Newcastle, Legnaro, PD, Italy
[2] Natl Vet Res Inst, Vom, Nigeria
[3] Natl Lab Qual Control Poultry Product, Cairo, Egypt
[4] Fogarty Int Ctr, NIH, Bethesda, MD USA
[5] Lab Cent Pathol Anim, Bingerville, Cote Ivoire
[6] Accra Vet Lab, Accra, Ghana
[7] Lab Cent Vet, Lome, Togo
[8] Lab Diagnost Vet Serosurveillance, Parakou, Benin
[9] Lab Natl Elevage, Ouagadougou, Burkina Faso
[10] Lab Natl Vet, Garoua, Cameroon
[11] Cent Vet Res Lab, Khartoum, Sudan
[12] Direct Lab Vet, Niamey, Niger
[13] Food & Agr Org UN, AGAH Div, Rome, Italy
来源
PLOS ONE | 2009年 / 4卷 / 03期
关键词
A VIRUSES; NEURAMINIDASE INHIBITORS; PCR ASSAY; HEMAGGLUTININ; EVOLUTION; BINDING; INTRODUCTIONS; MUTATIONS; NIGERIA; H7;
D O I
10.1371/journal.pone.0004842
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Highly pathogenic avian influenza virus A/H5N1 was first officially reported in Africa in early 2006. Since the first outbreak in Nigeria, this virus spread rapidly to other African countries. From its emergence to early 2008, 11 African countries experienced A/H5N1 outbreaks in poultry and human cases were also reported in three of these countries. At present, little is known of the epidemiology and molecular evolution of A/H5N1 viruses in Africa. We have generated 494 full gene sequences from 67 African isolates and applied molecular analysis tools to a total of 1,152 A/H5N1 sequences obtained from viruses isolated in Africa, Europe and the Middle East between 2006 and early 2008. Detailed phylogenetic analyses of the 8 gene viral segments confirmed that 3 distinct sublineages were introduced, which have persisted and spread across the continent over this 2-year period. Additionally, our molecular epidemiological studies highlighted the association between genetic clustering and area of origin in a majority of cases. Molecular signatures unique to strains isolated in selected areas also gave us a clearer picture of the spread of A/H5N1 viruses across the continent. Mutations described as typical of human influenza viruses in the genes coding for internal proteins or associated with host adaptation and increased resistance to antiviral drugs have also been detected in the genes coding for transmembrane proteins. These findings raise concern for the possible human health risk presented by viruses with these genetic properties and highlight the need for increased efforts to monitor the evolution of A/H5N1 viruses across the African continent. They further stress how imperative it is to implement sustainable control strategies to improve animal and public health at a global level.
引用
收藏
页数:9
相关论文
共 34 条
  • [1] CHARACTERIZATION OF INFLUENZA-A VIRUSES ISOLATED FROM TURKEYS IN ENGLAND DURING MARCH-MAY 1979
    ALEXANDER, DJ
    SPACKMAN, D
    [J]. AVIAN PATHOLOGY, 1981, 10 (03) : 281 - 293
  • [2] Diversity and evolution of West Nile virus in Illinois and the United States, 2002-2005
    Bertolotti, Luigi
    Kitron, Urlel
    Goldberg, Tony L.
    [J]. VIROLOGY, 2007, 360 (01) : 143 - 149
  • [3] Genomic signatures of human versus avian influenza a viruses
    Chen, Guang-Wu
    Chang, Shih-Cheng
    Mok, Chee-Keng
    Lo, Yu-Luan
    Kung, Yu-Nong
    Huang, Ji-Hung
    Shih, Yun-Han
    Wang, Ji-Yi
    Chiang, Chiayn
    Chen, Chi-Jene
    Shih, Shin-Ru
    [J]. EMERGING INFECTIOUS DISEASES, 2006, 12 (09) : 1353 - 1360
  • [4] Distribution of amantadine-resistant H5N1 avian influenza variants in Asia
    Cheung, Chung-Lam
    Rayner, Jane M.
    Smith, Gavin J. D.
    Wang, Pui
    Naipospos, T. S. P.
    Zhang, Jinxia
    Yuen, Kwok-Yung
    Webster, Robert G.
    Peiris, J. S. Malik
    Guan, Yi
    Chen, Honglin
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (12) : 1626 - 1629
  • [5] Molecular and antigenic evolution and geographical spread of H5N1 highly pathogenic avian influenza viruses in western Africa
    Ducatez, M. F.
    Olinger, C. M.
    Owoade, A. A.
    Tarnagda, Z.
    Tahita, M. C.
    Sow, A.
    De Landtsheer, S.
    Ammerlaan, W.
    Ouedraogo, J. B.
    Osterhaus, A. D. M. E.
    Fouchier, R. A. M.
    Muller, C. P.
    [J]. JOURNAL OF GENERAL VIROLOGY, 2007, 88 : 2297 - 2306
  • [6] Multiple introductions of H5N1 in Nigeria - Phylogenetic analysis reveals that this deadly virus first arrived in Africa from different sources
    Ducatez, M. F.
    Olinger, C. M.
    Owoade, A. A.
    De Landtsheer, S.
    Ammerlaan, W.
    Niesters, H. G. M.
    Osterhaus, A. D. M. E.
    Fouchier, R. A. M.
    Muller, C. P.
    [J]. NATURE, 2006, 442 (7098) : 37 - 37
  • [7] *EUR COMM, 2005, OFFICIAL J EUROPEA L, V10
  • [8] Mutations of neuraminidase implicated in neuraminidase inhibitors resistance
    Ferraris, Olivier
    Lina, Bruno
    [J]. JOURNAL OF CLINICAL VIROLOGY, 2008, 41 (01) : 13 - 19
  • [9] Persistent host markers in pandemic and H5N1 influenza viruses
    Finkelstein, David B.
    Mukatira, Suraj
    Mehta, Perdeep K.
    Obenauer, John C.
    Su, Xiaoping
    Webster, Robert G.
    Naeve, Clayton W.
    [J]. JOURNAL OF VIROLOGY, 2007, 81 (19) : 10292 - 10299
  • [10] Evolution of the receptor binding phenotype of influenza A (H5) viruses
    Gambaryan, A
    Tuzikov, A
    Pazynina, G
    Bovin, N
    Balish, A
    Klimov, A
    [J]. VIROLOGY, 2006, 344 (02) : 432 - 438