Characterization of VP7 and VP4 genes of rotavirus strains: 1990-1994 and 2000-2002

被引：21

作者：

Zade, J. K. ^{[1
]}

Chhabra, P. ^{[1
]}

Chitambar, S. D. ^{[1
]}

机构：

[1] Natl Inst Virol, Rotavirus Grp, Pune 411001, Maharashtra, India

来源：

EPIDEMIOLOGY AND INFECTION | 2009年 / 137卷 / 07期

关键词：

Rotavirus; unusual rotavirus strains; VP4; gene; VP7; SEROTYPE-SPECIFIC NEUTRALIZATION; POLYMERASE CHAIN-REACTION; SEQUENCE ALIGNMENT; GLOBAL COLLECTION; PORCINE ROTAVIRUS; DIVERSITY; CHILDREN; INDIA; GENOTYPE; VACCINE;

D O I：

10.1017/S0950268808001532

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n=77) and 2000-2002 (n=13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79-2% and 92-3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1-3% and 7-7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (GIP[6], GIP[4], GIP[19], G2P[8], G3P[4], G4P[6]) in 19-5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India.

引用

页码：936 / 942

页数：7

共 36 条

[1] Increased prevalence of G1P[4] genotype among children with rotavirus-associated gastroenteritis in metropolitan Detroit [J].

Abdel-Haq, NM ;

Thomas, RA ;

Asmar, BI ;

Zacharova, V ;

Lyman, WD .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) :2680-2682

[2] Further characterization of field strains of rotavirus from Nigeria VP4 genotype P6 most frequently identified among symptomatically infected children [J].

Adah, MI ;

Rohwedder, A ;

Olaleye, OD ;

Durojaiye, OA ;

Werchau, H .

JOURNAL OF TROPICAL PEDIATRICS, 1997, 43 (05) :267-274

[3] Changing patterns of rotavirus genotypes in Ghana: Emergence of human rotavirus G9 as a major cause of diarrhea in children [J].

Armah, GE ;

Steele, AD ;

Binka, FN ;

Esona, MD ;

Asmah, RH ;

Anto, F ;

Brown, D ;

Green, J ;

Cutts, F ;

Hall, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) :2317-2322

[4] Evidence of rotavirus AU32 like G9 strains from nontypeable fecal specimens of Indian children hospitalized during 1993-1994 [J].

Awachat, PS ;

Kelkar, SD .

JOURNAL OF MEDICAL VIROLOGY, 2004, 74 (04) :656-661

[5] Genetic variation in the VP7 gene of human rotavirus isolated in Montevideo-Uruguay from 1996-1999 [J].

Berois, M ;

Libersou, S ;

Russi, J ;

Arbiza, J ;

Cohen, J .

JOURNAL OF MEDICAL VIROLOGY, 2003, 71 (03) :456-462

[6]

BRUKE B, 1994, J GEN VIROL, V75, P2205

[7]

COOK SM, 1990, B WORLD HEALTH ORGAN, V68, P171

[8] RELATION OF VP7 AMINO-ACID-SEQUENCE TO MONOCLONAL-ANTIBODY NEUTRALIZATION OF ROTAVIRUS AND ROTAVIRUS MONOTYPE [J].

COULSON, BS ;

KIRKWOOD, C .

JOURNAL OF VIROLOGY, 1991, 65 (11) :5968-5974

[9]

DAS S, 2002, J MED VIROL, V72, P156

[10] LOCATION OF THE MAJOR ANTIGENIC SITES INVOLVED IN ROTAVIRUS SEROTYPE-SPECIFIC NEUTRALIZATION [J].

DYALLSMITH, ML ;

LAZDINS, I ;

TREGEAR, GW ;

HOLMES, IH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (10) :3465-3468

← 1 2 3 4 →