Characterization of VP7 and VP4 genes of rotavirus strains: 1990-1994 and 2000-2002

被引：21

作者：

Zade, J. K. ^{[1
]}

Chhabra, P. ^{[1
]}

Chitambar, S. D. ^{[1
]}

机构：

[1] Natl Inst Virol, Rotavirus Grp, Pune 411001, Maharashtra, India

来源：

EPIDEMIOLOGY AND INFECTION | 2009年 / 137卷 / 07期

关键词：

Rotavirus; unusual rotavirus strains; VP4; gene; VP7; SEROTYPE-SPECIFIC NEUTRALIZATION; POLYMERASE CHAIN-REACTION; SEQUENCE ALIGNMENT; GLOBAL COLLECTION; PORCINE ROTAVIRUS; DIVERSITY; CHILDREN; INDIA; GENOTYPE; VACCINE;

D O I：

10.1017/S0950268808001532

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n=77) and 2000-2002 (n=13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79-2% and 92-3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1-3% and 7-7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (GIP[6], GIP[4], GIP[19], G2P[8], G3P[4], G4P[6]) in 19-5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India.

引用

页码：936 / 942

页数：7

共 36 条

[21] Genetic and antigenic characterization of rotavirus serotype G9 strains isolated in Australia between 1997 and 2001 [J].

Kirkwood, C ;

Bogdanovic-Sakran, N ;

Palombo, E ;

Masendycz, P ;

Bugg, H ;

Barnes, G ;

Bishop, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3649-3654

[22] ANALYSIS OF THE NEWLY IDENTIFIED NEUTRALIZATION EPITOPES ON VP7 OF HUMAN ROTAVIRUS SEROTYPE-1 [J].

KOBAYASHI, N ;

TANIGUCHI, K ;

URASAWA, S .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :117-124

[23] MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].

Kumar, S ;

Tamura, K ;

Nei, M .

BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163

[24] Rotavirus G and P types circulating in Brazil: Characterization by RT-PCR, probe hybridization, and sequence analysis [J].

Leite, JPG ;

Alfieri, AA ;

Woods, PA ;

Glass, RI ;

Gentsch, JR .

ARCHIVES OF VIROLOGY, 1996, 141 (12) :2365-2374

[25] Full genome-based classification of rotaviruses reveals a common origin between human Wa-like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains [J].

Matthijnssens, Jelle ;

Ciarlet, Max ;

Heiman, Erica ;

Arijs, Ingrid ;

Delbeke, Thomas ;

McDonald, Sarah M. ;

Palombo, Enzo A. ;

Iturriza-Gomara, Miren ;

Maes, Piet ;

Patton, John T. ;

Rahman, Mustafizur ;

Van Ranst, Marc .

JOURNAL OF VIROLOGY, 2008, 82 (07) :3204-3219

[26]

Okada J, 2000, J MED VIROL, V60, P63, DOI 10.1002/(SICI)1096-9071(200001)60:1<63::AID-JMV11>3.0.CO

[27]

2-4

[28]

Palombo EA, 1999, FEMS MICROBIOL LETT, V181, P1

[29]

Parashar UD, 2006, EMERG INFECT DIS, V12, P304

[30] Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure [J].

Rahman, Mustafizur ;

Sultana, Rasheda ;

Podder, Goutam ;

Faruque, Abu S. G. ;

Matthijnssens, Jelle ;

Zaman, Khalequz ;

Breiman, Robert F. ;

Sack, David A. ;

Van Ranst, Marc ;

Azim, Tasnim .

VIROLOGY JOURNAL, 2005, 2 (1)

← 1 2 3 4 →