The calicheamicin gene cluster and its iterative type I enediyne PKS

被引：243

作者：

Ahlert, J

Shepard, E

Lomovskaya, N

Zazopoulos, E

Staffa, A

Bachmann, BO

Huang, KX

Fonstein, L

Czisny, A

Whitwam, RE

Farnet, CM

Thorson, JS

机构：

[1] Ecopia Biosci Inc, Montreal, PQ H4S 2A1, Canada

[2] Univ Wisconsin, Sch Pharm, Pharmaceut Sci Div, Lab Biosynthet Chem, Madison, WI 53705 USA

来源：

SCIENCE | 2002年 / 297卷 / 5584期

关键词：

D O I：

10.1126/science.1072105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

引用

页码：1173 / 1176

页数：5

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