A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing

被引:8
作者
Diego, LB
Berdasco, M
Fraga, MF
Cañal, MJ
Rodríguez, R
Castresana, C
机构
[1] Univ Oviedo, Fac Biol, Dept BOS, Lab Fisiol Vegetal, E-33071 Oviedo, Spain
[2] CSIC, Inst Biotecnol Asturias, E-33071 Oviedo, Spain
[3] Ctr Nacl Invest Oncol, Madrid, Spain
[4] Ctr Nacl, Lab Genet Mol Plantas, E-28049 Madrid, Spain
关键词
ageing; ATPase; expression; Pinus radiata; proteasome; proteolysis; RPT5a;
D O I
10.1093/jxb/erh171
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Age-dependent changes in gene expression profiles were studied in vegetative Pinus radiata buds by means of differential display. Among several candidate cDNAs, a 327 bp fragment that shows high homology with an Arabidopsis thaliana 20S proteasome ATPase designated RPT5a was found. Northern hybridization confirmed that the accumulation of this transcript increases with tree ageing, suggesting a possible role of this AAA-ATPase gene in development-related specific proteolysis.
引用
收藏
页码:1597 / 1599
页数:3
相关论文
共 13 条
[1]   Expression of a proteasome α-type subunit gene during tobacco development and senescence [J].
Bahrami, AR ;
Gray, JE .
PLANT MOLECULAR BIOLOGY, 1999, 39 (02) :325-333
[2]   Anatomical and biochemical events during in vitro rooting of microcuttings from juvenile and mature phases of chestnut [J].
Ballester, A ;
San-José, MC ;
Vidal, N ;
Fernández-Lorenzo, JL ;
Vietez, AM .
ANNALS OF BOTANY, 1999, 83 (06) :619-629
[3]   Changes in the expression and the enzymic properties of the 20S proteasome in sugar-starved maize roots. Evidence for an in vivo oxidation of the proteasome [J].
Basset, G ;
Raymond, P ;
Malek, L ;
Brouquisse, R .
PLANT PHYSIOLOGY, 2002, 128 (03) :1149-1162
[4]  
CALLIS J, 1995, PLANT CELL, V7, P845, DOI 10.1105/tpc.7.7.845
[5]   A reliable method for extraction of RNA from various conifer tissues [J].
Dong, JZ ;
Dunstan, DI .
PLANT CELL REPORTS, 1996, 15 (07) :516-521
[6]   Genomic DNA methylation-demethylation during aging and reinvigoration of Pinus radiata [J].
Fraga, MF ;
Rodríguez, R ;
Cañal, MJ .
TREE PHYSIOLOGY, 2002, 22 (11) :813-816
[7]  
Girod PA, 1999, PLANT CELL, V11, P1457
[8]   Ubiquitin- and proteasome-dependent proteolysis in plants [J].
Ingvardsen, C ;
Veierskov, B .
PHYSIOLOGIA PLANTARUM, 2001, 112 (04) :451-459
[9]  
LYNDON RF, 1998, SHOOT APICAL MERISTE, P194
[10]   Degradation of ornithine decarboxylase by the 26S proteasome [J].
Murakami, Y ;
Matsufuji, S ;
Hayashi, S ;
Tanahashi, N ;
Tanaka, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 267 (01) :1-6