Prediction of electrophoretic mobilities in non-aqueous capillary electrophoresis -: Optimal separation of quinolones in acetonitrile-water media

被引：41

作者：

Barrón, D ^{[1
]}

Irles, A ^{[1
]}

Barbosa, J ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 871卷 / 1-2期

关键词：

electrophoretic mobility; non-aqueous capillary electrophoresis; buffer composition; resolution prediction; quinolones; antibiotics;

D O I：

10.1016/S0021-9673(99)00858-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A model of electrophoretic behaviour is used to predict the optimum conditions for the separation of a series of quinolones zwitterionic substances in mixtures of acetonitrile-water up to 30% (w/w) of acetonitrile. The effect of pH, pK(a), the electrophoretic mobility of protonated and anionic species, and activity coefficients on the electrophoretic behaviour of quinolones is considered. The model proposed allows the resolution between substances in acetonitrile-water mixtures to be predicted from a few experimental data and thus permits one to obtain the best experimental conditions for separation methodologies. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：367 / 380

页数：14

共 42 条

[31] Analysis of antibiotics by liquid chromatography mass spectrometry
Niessen, WMA
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 812 (1-2) : 53 - 75
[32] REFERENCE VALUE STANDARDS AND PRIMARY STANDARDS FOR PH MEASUREMENTS IN ORGANIC-SOLVENTS AND WATER + ORGANIC-SOLVENT MIXTURES OF MODERATE TO HIGH PERMITTIVITIES
RONDININI, S
MUSSINI, PR
MUSSINI, T
[J]. PURE AND APPLIED CHEMISTRY, 1987, 59 (11) : 1549 - 1560
[33] AQUEOUS SOLUBILITIES OF SOME VARIOUSLY SUBSTITUTED QUINOLONE ANTIMICROBIALS
ROSS, DL
RILEY, CM
[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1990, 63 (03) : 237 - 250
[34] NONAQUEOUS CAPILLARY ELECTROPHORESIS
SAHOTA, RS
KHALEDI, MG
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (07) : 1141 - 1146
[35] Influence of organic solvents on the separation selectivity in capillary electrophoresis
Sarmini, K
Kenndler, E
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 792 (1-2) : 3 - 11
[36] SCHAEFER HG, 1995, INT J CLIN PHARM TH, V33, P266
[37] Optimization of capillary electrophoretic separation of quinolone antibacterials using the overlapping resolution mapping scheme
Sun, SW
Chen, LY
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 766 (1-2) : 215 - 224
[38] PROTONATION EQUILIBRIA OF QUINOLONE ANTIBACTERIALS
TAKACSNOVAK, K
NOSZAL, B
HERMECZ, I
KERESZTURI, G
PODANYI, B
SZASZ, G
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1990, 79 (11) : 1023 - 1028
[39] SIMULTANEOUS DETERMINATION OF ENROFLOXACIN AND ITS PRIMARY METABOLITE CIPROFLOXACIN IN BOVINE-MILK AND PLASMA BY ION-PAIRING LIQUID-CHROMATOGRAPHY
TYCZKOWSKA, KL
VOYKSNER, RD
ANDERSON, KL
PAPICH, MG
[J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 658 (02): : 341 - 348
[40] Study of 4-quinolone antibiotics in biological samples by short-column liquid chromatography coupled with electrospray ionization tandem mass spectrometry
Volmer, DA
Mansoori, B
Locke, SJ
[J]. ANALYTICAL CHEMISTRY, 1997, 69 (20) : 4143 - 4155

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