Glucocorticoid diminishes vascular endothelial growth factor and exacerbates proteinuria in rats with mesangial proliferative glomerulonephritis

被引:18
作者
Ha, IS
Um, EY
Jung, HR
Park, HW
Cheong, HI
Choi, Y
机构
[1] Seoul Natl Univ, Coll Med, Dept Pediat, Chongno Gu, Seoul 110744, South Korea
[2] Seoul Natl Univ Hosp, Clin Res Inst, Dept Pediat Nephrol, Seoul 110744, South Korea
[3] Seoul Natl Univ, Coll Med, Boramae Hosp, Dept Pediat, Seoul, South Korea
关键词
glucocorticoid; vascular endothelial growth factor (VEGF); proteinuria; mesangial proliferative glomerulonephritis; rat;
D O I
10.1053/ajkd.2002.32773
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Glucocorticoids are widely prescribed for renal diseases. It is believed that glucocorticolds attenuate immunemediated renal diseases by suppressing the cell-mediated immune system. However, there is evidence that glucocorticolds influence the expression of such growth factors as vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-beta1), and connective tissue growth factor (CTGF), which are known to influence the development or progression of renal diseases. Therefore, we undertook this study to determine whether glucocorticolds regulate proteinuria or extracellular matrix (ECM) production by altering these growth factors. Mesangial proliferative glomerulonephritis was induced in rats by intravenous injection of monoclonal antibody (OX-7), and dexamethasone (20 mg/kg) was administered intraperitoneally from the third to seventh disease day. Glomerular expression of VEGF, TGF-beta1, and CTGF, the amount of urinary protein, and glomerular ECM were measured on the seventh disease day. The nephritic group showed proteinuria and greater VEGF, TGF-beta1, and ECM production. Dexamethasone aggravated proteinuria (protein, 0.4 +/- 0.1 mg/mg creatinine in the NC group, 6.3 +/- 2.0 mg/mg creatinine in the DC group, and 21.1 +/- 1.9 mg/mg creatinine in the D-Dex group; P < 0.05) and diminished VEGF release (22 +/- 3 pg/mg total protein in the NC group, 292 +/- 26 pg/mg total protein in the DC group, and 198 +/- 23 pg/mg total protein in the D-Dex group; P < 0.05). Expression of TGF-beta1, CTGF, and ECM was not altered significantly by dexamethasone treatment. We found that glucocorticold diminishes VEGF release and at the same time exacerbates proteinuria in rats with this type of glomerulonephritis. (C) 2002 by the National Kidney Foundation, Inc.
引用
收藏
页码:1001 / 1010
页数:10
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