ROCK-II-induced membrane blebbing and chromatin condensation require actin cytoskeleton

被引：41

作者：

Song, YH ^{[1
]}

Hoang, BQ ^{[1
]}

Chang, DD ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Sch Med, Div Heme Onc, Dept Med Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

来源：

EXPERIMENTAL CELL RESEARCH | 2002年 / 278卷 / 01期

关键词：

Rho GTPase; apoptosis; tetracycline-inducible expression; actin filaments; cofilin; ML-7; caspase-3; z-VAD-fmk; MCF-7;

D O I：

10.1006/excr.2002.5565

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Ectopic expression of ROCK II (Rho kinase II or ROKalpha), an effector of Rho GTPase, induces membrane blebbing and chromatin condensation. ROCK 11 can induce membrane blebbing in the presence of the caspase inhibitor z-VAD-fmk or in caspase-3-deficient MCF-7 cells, indicating that the activation of caspases is not required. ROCK-II-induced membrane blebbing, however, is reversed by the myosin light chain kinase inhibitor ML-7 or cytochalasin D. In addition, the expression of a constitutively activated form of cofilin (S3A-cofilin) suppresses both membrane blebbing and chromatin condensation in ROCK II expressing cells. These findings suggest that the activation of actin-myosin contractility is responsible for membrane blebbing and chromatin condensation and implicate ROCK II as a potential mediator of the morphological changes associated with apoptosis. (C) 2002 Elsevier Science (USA).

引用

页码：45 / 52

页数：8

共 39 条

[11] Rho GTPases and the actin cytoskeleton [J].