Lipopolysaccharide-mediated cell activation without rapid mobilization of cytosolic free calcium

被引：18

作者：

Bosshart, H

Heinzelmann, M

机构：

[1] Univ Zurich Hosp, Dept Surg, CH-8091 Zurich, Switzerland

[2] Zurich Univ Hosp, Dept Orthoped Surg, CH-8008 Zurich, Switzerland

[3] Zurich Univ Hosp, Dept Med, CH-8008 Zurich, Switzerland

[4] Dept Psychiat, CH-8029 Zurich, Switzerland

来源：

MOLECULAR IMMUNOLOGY | 2004年 / 41卷 / 10期

关键词：

gram-negative endotoxin; pattern recognition receptor; calcium mobilization; cytokine release;

D O I：

10.1016/j.molimm.2004.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The involvement of cytosolic free calcium (Ca2+) as a second messenger in lipopolysaccharide (LPS)-activated cellular pathways remains controversial. Using human monocyte-like THP-1 cells stably transfected with glycosylphosphatidylinositol-anchored human CD14, we have monitored the concentration of intracellular Ca2+ after sequential addition of LPS, ATP, and ionomycin. Whereas ATP and ionomycin induced rapid elevations of cytosolic Ca2+, no such changes were observed after LPS addition. These data argue against the participation of cytosolic Ca2+ in the CD14-dependent LPS activation pathway. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1023 / 1028

页数：6

共 25 条

[1] Plasma membrane calcium pump: Structure, function and relationships [J].