Combinatorial expression of TRPV channel proteins defines their sensory functions and subcellular localization in C-elegans neurons

被引:327
作者
Tobin, DM
Madsen, DM
Kahn-Kirby, A
Peckol, EL
Moulder, G
Barstead, R
Maricq, AV
Bargmann, CI [1 ]
机构
[1] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Program Dev Biol, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Genet Program, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Program Neurosci, San Francisco, CA 94143 USA
[5] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA
[6] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
[7] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA
[8] Oklahoma Med Res Fdn, Oklahoma City, OK 73104 USA
基金
美国国家科学基金会;
关键词
D O I
10.1016/S0896-6273(02)00757-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
C. elegans OSM-9 is a TRPV channel protein involved in sensory transduction and adaptation. Here, we show that distinct sensory functions arise from different combinations of OSM-9 and related OCR TRPV proteins. Both OSM-9 and OCR-2 are essential for several forms of sensory transduction, including olfaction, osmosensation, mechanosensation, and chemosensation. In neurons that express both OSM-9 and OCR-2, tagged OCR-2 and OSM-9 proteins reside in sensory cilia and promote each other's localization to cilia. In neurons that express only OSM-9, tagged OSM-9 protein resides in the cell body and acts in sensory adaptation rather than sensory transduction. Thus, alternative combinations of TRPV proteins may direct different functions in distinct subcellular locations. Animals expressing the mammalian TRPV1 (VR1) channel in ASH nociceptor neurons avoid the TRPV1 ligand capsaicin, allowing selective, drug-inducible activation of a specific behavior.
引用
收藏
页码:307 / 318
页数:12
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