KOBITO1 encodes a novel plasma membrane protein necessary for normal synthesis of cellulose during cell expansion in Arabidopsis

被引:129
作者
Pagant, S
Bichet, A
Sugimoto, K
Lerouxel, O
Desprez, T
McCann, M
Lerouge, P
Vernhettes, S
Höfte, H
机构
[1] INRA, Biol Cellulaire Lab, F-78026 Versailles, France
[2] John Innes Ctr, Dept Cell & Dev Biol, Norwich NR4 7UH, Norfolk, England
[3] Univ Sci, CNRS, UMR, IFRMP23, F-76821 Mont St Aignan, France
关键词
D O I
10.1105/tpc.002873
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cell wall is the major limiting factor for plant growth. Wall extension is thought to result from the loosening of its structure. However, it is not known how this is coordinated with wall synthesis. We have identified two novel allelic cellulose-deficient dwarf mutants, kobito1-1 and kobito1-2 (kob1-1 and kob1-2). The cellulose deficiency was confirmed by the direct observation of microfibrils in most recent wall layers of elongating root cells. In contrast to the wild type, which showed transversely oriented parallel microfibrils, kob1 microfibrils were randomized and occluded by a layer of pectic material. No such changes were observed in another dwarf mutant, pom1, suggesting that the cellulose defect in kob1 is not an indirect result of the reduced cell elongation. Interestingly, in the meristematic zone of kob1 roots, microfibrils appeared unaltered compared with the wild type, suggesting a role for KOB1 preferentially in rapidly elongating cells. KOB1 was cloned and encodes a novel, highly conserved, plant-specific protein that is plasma membrane bound, as shown with a green fluorescent protein-KOB1 fusion protein. KOB1 mRNA was present in all organs investigated, and its overexpression did not cause visible phenotypic changes. KOB1 may be part of the cellulose synthesis machinery in elongating cells, or it may play a role in the coordination between cell elongation and cellulose synthesis.
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页码:2001 / 2013
页数:13
相关论文
共 36 条
[21]   Effects of the mur1 mutation on xyloglucans produced by suspension-cultured Arabidopsis thaliana cells [J].
Pauly, M ;
Eberhard, S ;
Albersheim, P ;
Darvill, A ;
York, WS .
PLANTA, 2001, 214 (01) :67-74
[22]   Fractionation of carbohydrates in Arabidopsis root cell walls shows that three radial swelling loci are specifically involved in cellulose production [J].
Peng, LC ;
Hocart, CH ;
Redmond, JW ;
Williamson, RE .
PLANTA, 2000, 211 (03) :406-414
[23]  
RICHMOND T, 2000, GENOME BIOL, V1
[24]   Role of the putative membrane-bound endo-1,4-β-glucanase KORRIGAN in cell elongation and cellulose synthesis in Arabidopsis thaliana [J].
Sato, S ;
Kato, T ;
Kakegawa, K ;
Ishii, T ;
Liu, YG ;
Awano, T ;
Takabe, K ;
Nishiyama, Y ;
Kuga, S ;
Sato, S ;
Nakamura, Y ;
Tabata, S ;
Shibata, D .
PLANT AND CELL PHYSIOLOGY, 2001, 42 (03) :251-263
[25]   Wall architecture in the cellulose-deficient rsw1 mutant of Arabidopsis thaliana:: microfibrils but not microtubules lose their transverse alignment before microfibrils become unrecognizable in the mitotic and elongation zones of roots [J].
Sugimoto, K ;
Williamson, RE ;
Wasteneys, GO .
PROTOPLASMA, 2001, 215 (1-4) :172-183
[26]   New techniques enable comparative analysis of microtubule orientation, wall texture, and growth rate in intact roots of Arabidopsis [J].
Sugimoto, K ;
Williamson, RE ;
Wasteneys, GO .
PLANT PHYSIOLOGY, 2000, 124 (04) :1493-1506
[27]   The irregular xylem3 locus of arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis [J].
Taylor, NG ;
Scheible, WR ;
Cutler, S ;
Somerville, CR ;
Turner, SR .
PLANT CELL, 1999, 11 (05) :769-779
[28]   Multiple cellulose synthase catalytic subunits are required for cellulose synthesis in Arabidopsis [J].
Taylor, NG ;
Laurie, S ;
Turner, SR .
PLANT CELL, 2000, 12 (12) :2529-2539
[29]   Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall [J].
Turner, SR ;
Somerville, CR .
PLANT CELL, 1997, 9 (05) :689-701
[30]   SEMIMICRO DETERMINATION OF CELLULOSE IN BIOLOGICAL MATERIALS [J].
UPDEGRAFF, DM .
ANALYTICAL BIOCHEMISTRY, 1969, 32 (03) :420-+