Bilirubin inhibits the TNFα-related induction of three endothelial adhesion molecules

被引：99

作者：

Mazzone, Graciela Lujan ^{[1
]}

Rigato, Igino ^{[1
,3
]}

Ostrow, J. Donald ^{[4
,5
]}

Bossi, Fleur ^{[2
]}

Bortoluzzi, Alessia ^{[1
]}

Sukowati, Caecilia H. C. ^{[1
]}

Tedesco, Francesco ^{[2
]}

Tiribelli, Claudio ^{[1
]}

机构：

[1] Ctr Studi Fegato, I-34012 Trieste, Italy

[2] Univ Trieste, Dept Life Sci, I-34127 Trieste, Italy

[3] San Vito Tagliamento Hosp, Emergency Dept, I-33078 San Vito Al Tagliamento, PN, Italy

[4] Univ Washington, Div Gastroenterol Hepatol, Seattle, WA 98108 USA

[5] Vet Affairs Med Ctr, Seattle, WA 98108 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 386卷 / 02期

关键词：

Endothelial cell activation; Bilirubin; Adhesion molecules; TNF alpha; Atherosclerosis; ATHEROSCLEROSIS; SERUM; CELLS; PATHOGENESIS; INFLAMMATION; RECRUITMENT; DISEASE; ALBUMIN;

D O I：

10.1016/j.bbrc.2009.06.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Since an increased serum unconjugated bilirubin (UCB) level has been proposed as an independent protective factor against atherosclerotic disease, we investigated the molecular events at the basis of this effect. HUVEC and H5V cells were treated with TNF alpha and UCB and the effects assessed on E-selectin, VCAM-1 and ICAM-1. In HUVEC cells, UCB blunted the TNF alpha-induced gene upregulation of E-selectin VCAM-1 and ICAM-1. The same pattern was observed in H5V cells except for ICAM-1. UCB also inhibited the PMN endothelial adhesion in HUVEC H5V cells. Western blot and FACS analysis confirmed that UCB prevented TNF alpha-induced over-expression of adhesion molecules proteins in H5V cells. These data contribute to further explain the protective effect of bilirubin against development of atherosclerosis. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：338 / 344

页数：7

共 20 条

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