Wogonoside inhibits lipopolysaccharide-induced angiogenesis in vitro and in vivo via toll-like receptor 4 signal transduction

被引：57

作者：

Chen, Yan ^{[1
]}

Lu, Na ^{[1
]}

Ling, Yun ^{[1
]}

Gao, Ying ^{[1
]}

Wang, Ling ^{[1
]}

Sun, Yu ^{[1
]}

Qi, Qi ^{[1
]}

Feng, Feng ^{[1
]}

Liu, Wenyuan ^{[2
]}

Liu, Wei ^{[1
]}

You, Qidong ^{[1
]}

Guo, Qinglong ^{[1
]}

机构：

[1] China Pharmaceut Univ, Jiangsu Key Lab Carcinogenesis & Intervent, Nanjing 210009, Peoples R China

[2] China Pharmaceut Univ, Dept Pharmaceut Anal, Nanjing 210009, Peoples R China

来源：

TOXICOLOGY | 2009年 / 259卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Wogonoside; Lipopolysaccharide; Angiogenesis; Toll-like receptor 4; SCUTELLARIA-BAICALENSIS GEORGI; ENDOTHELIAL-GROWTH-FACTOR; NF-KAPPA-B; TYROSINE PHOSPHORYLATION; BACTERIAL LIPOPOLYSACCHARIDE; TUMOR-GROWTH; CELLS; WOGONIN; EXPRESSION; RADIX;

D O I：

10.1016/j.tox.2009.01.010

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Wogonoside, one flavonoid derived from the root of Scutellaria baicalensis Georgi, has been reported for its anti-inflammation activity; however, whether it can inhibit inflammation-induced angiogenesis is still unclear. In the present study, we evaluated the effect of wogonoside on lipopolysaccharide (LPS)-induced angiogenesis in vitro and in vivo. Wogonoside suppressed the LPS-stimulated migration and tube formation of human umbilical vein endothelial cells (HUVECs), as well as microvessel sprouting from Fat aortic rings in vitro. Moreover, wogonoside also inhibited LPS-stimulated vessel growth of Chicken chorioallantoic membrane (CAM) in vivo. The mechanism revealed that wogonoside inhibited LPS-induced toll-like receptor 4 (TLR4) up-regulation and its downstream mitogen-activated protein kinases (MAPKs) activation, by decreasing the phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase. The results suggest that wogonoside inhibits LPS-induced angiogenesis both in vitro and in vivo, and that it might have a therapeutic potential for the diseases associated with the development of both inflammation and angiogenesis progress. (C) 2009 Elsevier Ireland Ltd. All Fights reserved.

引用

页码：10 / 17

页数：8

共 35 条

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