The chAB4 and NF1-related long-range multisequence DNA families are contiguous in the centromeric heterochromatin of several human chromosomes

被引：6

作者：

Cserpán, I ^{[1
]}

Katona, R ^{[1
]}

Praznovszky, T ^{[1
]}

Novák, E ^{[1
]}

Rózsavölgyi, M ^{[1
]}

Csonka, E ^{[1
]}

Mórocz, M ^{[1
]}

Fodor, K ^{[1
]}

Hadlaczky, G ^{[1
]}

机构：

[1] Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 13期

关键词：

D O I：

10.1093/nar/gkf382

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the large-scale organization of the human chAB4-related long-range multisequence family, a low copy-number repetitive DNA located in the pericentromeric heterochromatin of several human chromosomes. Analysis of genomic clones revealed large-scale (similar to100 kb or more) sequence conservation in the region flanking the prototype chAB4 element. We demonstrated that this low copy-number family is connected to another long-range repeat, the NF1-related (PsiNF1) multisequence. The two DNA types are joined by an similar to2 kb-long tandem repeat of a 48-bp satellite. Although the chAB4- and NF1-like sequences were known to have essentially the same chromosomal localization, their close association is reported here for the first time. It indicates that they are not two independent long-range DNA families, but are parts of a single element spanning similar to200 kb or more. This view is consistent both with their similar chromosomal localizations and the high levels of sequence conservation among copies found on different chromosomes. We suggest that the master copy of the linked chAB4-PsiNF1 DNA segment appeared first on the ancestor of human chromosome 17.

引用

页码：2899 / 2905

页数：7

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