Sequence of DNA 16S/23S spacer region of Leuconostoc oenos (OEnococcus oeni): Application to strain differentiation

被引：37

作者：

LeJeune, C ^{[1
]}

LonvaudFunel, A ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,INRA,INST OENOL,UNITE ASSOCIEE,F-33405 TALENCE,FRANCE

来源：

RESEARCH IN MICROBIOLOGY | 1997年 / 148卷 / 01期

关键词：

PCR; tRNA; Leuconostoc oe nos; OE nococcus oe ni; strain differentiation; rDNA; wine-making;

D O I：

10.1016/S0923-2508(97)81903-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Leuconostoc oenos is involved in malolactic fermentation occurring during wine-making. An increasing number of wines are being inoculated with malolactic starters to control the process, and the identification and differentiation of selected strains are now indispensable both for quality control of production and for commercial purposes. In the present work we evaluated the potential use of the intergenic regions of three L. oenos strains for their differentiation. The three 16S/23S rRNA intergenic spacers were amplified in vitro by PCR, and sequences were compared. The spacer sequence was highly conserved in all strains. Inside this spacer, a tRNA-Ala gene containing an 18-bp sequence stretch which is conserved in all tRNA genes was discovered. This sequence, together with random primers, was used for characterization of ten L. oenos strains by PCR.

引用

页码：79 / 86

页数：8

共 22 条

[1] NOVEL TRANSFER-RNA GENE ORGANIZATION IN THE 16S-23S INTERGENIC SPACER OF THE STREPTOCOCCUS-PNEUMONIAE RIBOSOMAL-RNA GENE-CLUSTER [J].

BACOT, CM ;

REEVES, RH .

JOURNAL OF BACTERIOLOGY, 1991, 173 (13) :4234-4236

[2]

BERGEY, 1986, MANUAL DETERMINATIVE

[3]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V274, P398

[4]

BROSIUS J, 1978, P NATL ACAD SCI USA, V75, P4801, DOI 10.1073/pnas.75.10.4801

[5] OPTIMIZATION OF TRANSVERSE ALTERNATING-FIELD ELECTROPHORESIS FOR STRAIN IDENTIFICATION OF LEUCONOSTOC-OENOS [J].

DANIEL, P ;

DEWAELE, E ;

HALLET, JN .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1993, 38 (05) :638-641

[6] PROPOSAL TO RECLASSIFY LEUCONOSTOC-OENOS AS OENOCOCCUS-OENI [CORRIG] GEN-NOV, COMB-NOV [J].

DICKS, LMT ;

DELLAGLIO, F ;

COLLINS, MD .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (02) :395-397

[7]

DOWER WJ, 1988, NUCLEIC ACIDS RES, V16, P13

[8] LOCALIZATION AND STRUCTURAL-ANALYSIS OF THE RIBOSOMAL-RNA OPERONS OF RHODOBACTER-SPHAEROIDES [J].

DRYDEN, SC ;

KAPLAN, S .

NUCLEIC ACIDS RESEARCH, 1990, 18 (24) :7267-7277

[9] CONJUGAL TRANSFER OF THE DRUG-RESISTANCE PLASMID PAM-BETA IN THE LACTIC STREPTOCOCCI [J].

GASSON, MJ ;

DAVIES, FL .

FEMS MICROBIOLOGY LETTERS, 1980, 7 (01) :51-53

[10] ARE POINT MUTATIONS OR DNA REARRANGEMENTS RESPONSIBLE FOR THE RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS THAT ARE USED TO TYPE BACTERIA [J].

HALL, LMC .

MICROBIOLOGY-UK, 1994, 140 :197-204

← 1 2 3 →