Multifunctional Bioconjugate for Cancer Cell-Targeted Theranostics

被引：25

作者：

Du, Wei ^{[1
]}

Yuan, Yue ^{[1
]}

Wang, Lin ^{[3
]}

Cui, Yusi ^{[1
]}

Wang, Hui ^{[2
]}

Xu, Huiqin ^{[2
]}

Liang, Gaolin ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Chem, Natl Synchrotron Radiat Lab, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China

[2] Anhui Med Univ, Affiliated Hosp 1, Dept Nucl Med, Hefei 230022, Anhui, Peoples R China

[3] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China

来源：

BIOCONJUGATE CHEMISTRY | 2015年 / 26卷 / 12期

基金：

中国国家自然科学基金;

关键词：

DRUG-DELIVERY; OLIGOMERIC NANOPARTICLES; TUMOR-MARKER; QUANTUM DOTS; APTAMER; MUC1; DOXORUBICIN;

D O I：

10.1021/acs.bioconjchem.5b00570

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Cancer cell-targeted imaging and drug delivery remain a challenge for precise cancer theranostics. MUC1 is a large transmembrane glycoprotein that may potentially serve as a target for cancer theranostics. Herein, using a MUC1-targeting aptamer (APT) as the "warhead", we rationally designed and constructed a hybrid nanoparticle 1-NPs-QDs-hAPT (Vehicle) that could be applied for MUC1-targeted cell uptake and imaging. By intercalating different Vehicle amounts with the anticancer drug doxorubicin (DOX), we obtained the multifunctional bioconjugate Vehicle-DOX with a maximized drug payload and DOX fluorescence quenching capability. Confocal microscopy cell imaging indicated that Vehicle-DOX could be used to track MUC1-targeted drug release. A cytotoxicity study indicated that Vehicle-DOX could be applied for MUC1-targeted cytotoxicity. We anticipate that our multifunctional bioconjugate Vehicle-DOX could be applied for in vivo tumor-targeted theranostics.

引用

页码：2571 / 2578

页数：8

共 41 条

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