Ultrarapid Detection of Pathogenic Bacteria Using a 3D Immunomagnetic Flow Assay

被引：95

作者：

Lee, Wonjae ^{[1
]}

Kwon, Donghoon ^{[1
]}

Chung, Boram ^{[2
]}

Jung, Gyoo Yeol ^{[1
,2
]}

Au, Anthony ^{[3
]}

Folch, Albert ^{[3
]}

Jeon, Sangmin ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Dept Chem Engn, Pohang, South Korea

[2] Pohang Univ Sci & Technol POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang, South Korea

[3] Univ Washington, Dept Bioengn, Seattle, WA 98105 USA

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 13期

基金：

新加坡国家研究基金会;

关键词：

SEPARATION;

D O I：

10.1021/ac501436d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We developed a novel 3D immunomagnetic flow assay for the rapid detection of pathogenic bacteria in a large-volume food sample. Antibody-functionalized magnetic nanoparticle clusters (AbMNCs) were magnetically immobilized on the surfaces of a 3D-printed cylindrical microchannel. The injection of a Salmonella-spiked sample solution into the microchannel produced instant binding between the AbMNCs and the Salmonella bacteria due to their efficient collisions. Nearly perfect capture of the AbMNCs and AbMNCs-Salmonella complexes was achieved under a high flow rate by stacking permanent magnets with spacers inside the cylindrical separator to maximize the magnetic force. The concentration of the bacteria in solution was determined using ATP luminescence measurements. The detection limit was better than 10 cfu/mL, and the overall assay time, including the binding, rinsing, and detection steps for a 10 mL sample took less than 3 min. To our knowledge, the 3D immunomagnetic flow assay described here provides the fastest high-sensitivity, high-capacity method for the detection of pathogenic bacteria.

引用

页码：6683 / 6688

页数：6

共 15 条

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