Engineering RNA sequence specificity of Pumilio repeats

被引：184

作者：

Cheong, Cheorn-Gil ^{[1
]}

Hall, Traci M. Tanaka ^{[1
]}

机构：

[1] Natl Inst Environm Hlth Sci, Lab Struct Biol, NIH, Res Triangle Pk, NC 27709 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 37期

关键词：

protein design; Puf proteins; protein-RNA interaction; adenosine-uracil-rich elements;

D O I：

10.1073/pnas.0606294103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence specificity, including one that binds tightly to adenosine-uracil-rich element RNA. These data show that Pumilio1 can be used as a scaffold to engineer RNA-binding proteins with designed sequence specificity.

引用

页码：13635 / 13639

页数：5

共 41 条

[1] CONTROL OF THE SPERM-OOCYTE SWITCH IN CAENORHABDITIS-ELEGANS HERMAPHRODITES BY THE FEM-3 3' UNTRANSLATED REGION [J].

AHRINGER, J ;

KIMBLE, J .

NATURE, 1991, 349 (6307) :346-348

[2] Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos [J].

Asaoka-Taguchi, M ;

Yamada, M ;

Nakamura, A ;

Hanyu, K ;

Kobayashi, S .

NATURE CELL BIOLOGY, 1999, 1 (07) :431-437

[3] PUMILIO IS ESSENTIAL FOR FUNCTION BUT NOT FOR DISTRIBUTION OF THE DROSOPHILA ABDOMINAL DETERMINANT NANOS [J].

BARKER, DD ;

WANG, C ;

MOORE, J ;

DICKINSON, LK ;

LEHMANN, R .

GENES & DEVELOPMENT, 1992, 6 (12A) :2312-2326

[4] Structure-based design of a dimeric RNA-peptide complex [J].

Campisi, DM ;

Calabro, V ;

Frankel, AD .

EMBO JOURNAL, 2001, 20 (1-2) :178-186

[5] IDENTIFICATION OF A COMMON NUCLEOTIDE-SEQUENCE IN THE 3'-UNTRANSLATED REGION OF MESSENGER-RNA MOLECULES SPECIFYING INFLAMMATORY MEDIATORS [J].

CAPUT, D ;

BEUTLER, B ;

HARTOG, K ;

THAYER, R ;

BROWNSHIMER, S ;

CERAMI, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (06) :1670-1674

[6] Feedback inhibition of macrophage tumor necrosis factor-α production by tristetraprolin [J].

Carballo, E ;

Lai, WS ;

Blackshear, PJ .

SCIENCE, 1998, 281 (5379) :1001-1005

[7] Ab initio interaction energies of hydrogen-bonded amino acid side chain-nucleic acid base interactions [J].

Cheng, AC ;

Frankel, AD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (02) :434-435

[8] A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans [J].

Crittenden, SL ;

Bernstein, DS ;

Bachorik, JL ;

Thompson, BE ;

Gallegos, M ;

Petcherski, AG ;

Moulder, G ;

Barstead, R ;

Wickens, M ;

Kimble, J .

NATURE, 2002, 417 (6889) :660-663

[9] The staufen/pumilio pathway is involved in Drosophila long-term memory [J].

Dubnau, J ;

Chiang, AS ;

Grady, L ;

Barditch, J ;

Gossweiler, S ;

McNeil, J ;

Smith, P ;

Buldoc, F ;

Scott, R ;

Certa, U ;

Broger, C ;

Tully, T .

CURRENT BIOLOGY, 2003, 13 (04) :286-296

[10] Structure of Pumilio reveals similarity between RNA and peptide binding motifs [J].

Edwards, TA ;

Pyle, SE ;

Wharton, RP ;

Aggarwal, AK .

CELL, 2001, 105 (02) :281-289

← 1 2 3 4 5 →