Separation of leucine and isoleucine by electrospray ionization-high field asymmetric waveform ion mobility spectrometry-mass spectrometry

被引：107

作者：

Barnett, DA

Ells, B

Guevremont, R ^{[1
]}

Purves, RW

机构：

[1] Natl Res Council Canada, Inst Natl Measurement Stand, Ottawa, ON K1A 0R6, Canada

[2] PE SCIEX, Concord, ON, Canada

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1999年 / 10卷 / 12期

关键词：

D O I：

10.1016/S1044-0305(99)00098-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrospray ionization (ESI) source was used to generate gas-phase molecular anions of the amino acids leucine and isoleucine ((M-H)(-); m/z -130), which were separated by high-field asymmetric waveform ion mobility spectrometry (FAIMS) and detected by quadrupole mass spectrometry (MS). This combination of ESI-FAIMS-MS enabled selective determination of either amino acid in mixtures that contained at least a 625-fold excess of the other. Comparisons with conventional ESI-MS showed a 50-fold improvement in the signal to background ratio for a 1 mu M solution of leucine. (C) 1999 American Society for Mass Spectrometry.

引用

页码：1279 / 1284

页数：6

共 25 条

[1]

AUBAGNAC L, 1985, ORG MASS SPECTROM, V20, P428

[2] FAST ATOM BOMBARDMENT MASS-SPECTROMETRY OF THE ANGIOTENSIN PEPTIDES [J].