Exosomal circLPAR1 Promoted Osteogenic Differentiation of Homotypic Dental Pulp Stem Cells by Competitively Binding to hsa-miR-31

被引：59

作者：

Xie, Liangkun ^{[1
,2
]}

Guan, Zheng ^{[3
]}

Zhang, Mingzhu ^{[4
]}

Lyu, Sha ^{[3
]}

Thuaksuban, Nattawut ^{[2
]}

Kamolmattayakul, Suttatip ^{[5
]}

Nuntanaranont, Thongchai ^{[2
]}

机构：

[1] Kunming Med Univ, Dept Oral Implantol, Affiliated Stomatol Hosp, Kunming, Yunnan, Peoples R China

[2] Prince Songkla Univ, Dept Oral & Maxillofacial Surg, Fac Dent, Hat Yai, Songkhla, Thailand

[3] Kunming Med Univ, Biomed Res Ctr, Affiliated Calmette Hosp, First Hosp Kunming, Kunming, Yunnan, Peoples R China

[4] Kunming Med Univ, Dept Periodontol, Affiliated Stomatol Hosp, Kunming, Yunnan, Peoples R China

[5] Bangkok Thonburi Univ, Fac Dent, Bangkok, Thailand

来源：

BIOMED RESEARCH INTERNATIONAL | 2020年 / 2020卷

基金：

中国国家自然科学基金;

关键词：

MICRORNA EXPRESSION; STROMAL CELLS; IN-VITRO; MIR-31; ABUNDANT; REVEALS; SATB2;

D O I：

10.1155/2020/6319395

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Human dental pulp stem cells (DPSCs) hold great promise in bone regeneration. However, the exact mechanism of osteogenic differentiation of DPSCs remains unknown, especially the role of exosomes played in. The DPSCs were cultured and received osteogenic induction; then, exosomes from osteogenic-induced DPSCs (OI-DPSC-Ex) at different time intervals were isolated and sequenced for circular RNA (circRNA) expression profiles. Gradually, increased circular lysophosphatidic acid receptor 1 (circLPAR1) expression was found in the OI-DPSC-Ex coincidentally with the degree of osteogenic differentiation. Meanwhile, results from osteogenic differentiation examinations showed that the OI-DPSC-Ex had osteogenic effect on the recipient homotypic DPSCs. To investigate the mechanism of exosomal circLPAR1 on osteogenic differentiation, we verified that circLPAR1 could competently bind to hsa-miR-31, by eliminating the inhibitory effect of hsa-miR-31 on osteogenesis, therefore promoting osteogenic differentiation of the recipient homotypic DPSCs. Our study showed that exosomal circRNA played an important role in osteogenic differentiation of DPSCs and provided a novel way of utilization of exosomes for the treatment of bone deficiencies.

引用

页数：13

共 37 条

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MicroRNA expression profiling of human bone marrow mesenchymal stem cells during osteogenic differentiation reveals Osterix regulation by miR-31 [J].